plus 0.98 is deamidation
#hplc-massspec 2025-01-15
Wednesday11 messages2 participantstimes are UTC
Highlights from this day
- monoisotopic — a shoulder on a peak is information, and usually bad information 21:21
- salt_bridge — how do you spot a deletion in a sequence from mass alone 21:45
- salt_bridge — two labs a point or two apart on the same lot is normal and gets mistaken for one of them being wrong constantly, your mileage will differ 22:15
- monoisotopic — 214nm sees the amide backbone so it sees everything. 280 only sees aromatics, from memory 22:17
a shoulder on a peak is information, and usually bad information
how do you tell co-elution from a clean single peak
ms/ms or it isnt identity
ask for the trace
do the arithmetic
💀4📈17🧪3
how do you spot a deletion in a sequence from mass alone
two labs a point or two apart on the same lot is normal and gets mistaken for one of them being wrong constantly, your mileage will differ
214nm sees the amide backbone so it sees everything. 280 only sees aromatics, from memory
within inter-lab range
retention time is a hint