i was wrong about tailing factor. it is usually the injection or the pH, not the column
🎉9
#hplc-massspec 2025-07-24
Thursday11 messages4 participantstimes are UTC
Highlights from this day
- mg_per_ml — i was wrong about tailing factor. it is usually the injection or the pH, not the column 11:17
- LC_MS_Lena — why do two labs integrate the same peak differently 11:29
- LC_MS_Lena — if the number you are staring at is a third of what you expected, do the charge state arithmetic before you panic, ill dig out the number 11:31
- LC_MS_Lena — nobody runs enough blanks, including me 11:52
within inter-lab range
why do two labs integrate the same peak differently
👀2📈1
if the number you are staring at is a third of what you expected, do the charge state arithmetic before you panic, ill dig out the number
whats a normal gradient for a peptide this size
show me the spectrum
fridge-temps.csv
534 rows · not retained in the public archive
nobody runs enough blanks, including me
⚠️7👍12🤝1
need more sample
❤️11
thats the solvent peak
ask for the trace
🙏4
is there any point running a blank if the sample is the question