truncations show as a mass deficit matching one or more residues. you need the sequence to say which
#hplc-massspec 2025-10-22
Wednesday11 messages3 participantstimes are UTC
Highlights from this day
- noct.titrate — two labs a point or two apart on the same lot is normal and gets mistaken for one of them being wrong constantly 20:56
- noct.titrate — while im here is monoisotopic or average mass the one on most certificates 21:03
- noct.titrate — unrelated but is there any point running a blank if the sample is the question 21:04
coming back after 19 months, what changed in how you read these
two labs a point or two apart on the same lot is normal and gets mistaken for one of them being wrong constantly
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retention time is a hint, not a fingerprint. two different peptides can co-elute perfectly happily
plus sixteen, is that always oxidation
while im here is monoisotopic or average mass the one on most certificates
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unrelated but is there any point running a blank if the sample is the question
lot-log.csv
337 rows · not retained in the public archive
nobody runs enough blanks, including me
if somebody says they ran a mass spec and does not show you a spectrum, that is a claim, not a result
how much sample does a lab actually need for identity as well as purity
how do you spot a deletion in a sequence from mass alone