a certificate with a number and no chromatogram is asking you to trust the integration you cannot see
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#hplc-massspec 2026-01-18
Sunday13 messages4 participantstimes are UTC
Highlights from this day
- peak_split — a certificate with a number and no chromatogram is asking you to trust the integration you cannot see 10:55
- peak_split — if somebody says they ran a mass spec and does not show you a spectrum, that is a claim, not a result 11:24
- no_escrow_nate — shallow gradients hide related substances behind the main peak. a steeper one separates and looks worse, honestly, still working it out 11:27
- first_month_fi — whats the void volume peak, is that always solvent 13:45
thats a charge state
ask for the trace
integration choices move the number by a few tenths easily. that is most of your inter-lab variance, happy to be corrected
how would a truncation sequence show up
if somebody says they ran a mass spec and does not show you a spectrum, that is a claim, not a result
🤝2
genuine question how do you spot a deletion in a sequence from mass alone
nobody runs enough blanks, including me
shallow gradients hide related substances behind the main peak. a steeper one separates and looks worse, honestly, still working it out
whats the void volume peak, is that always solvent
⚠️18🙏1📉1
deamidation is about plus 0.98 Da. it is not a rounding error, it is a degradant
has anyone ever had a result where the mass was right and the purity was wrong
coming back after 26 months, what changed in how you read these