does retention time on its own tell you what the compound is
co-elution is the failure mode that a beautiful single peak is most likely to be hiding
#hplc-massspec 2026-06-13
Saturday14 messages4 participantstimes are UTC
Highlights from this day
- ms_ms_mira — does retention time on its own tell you what the compound is co-elution is the failure mode that a beautiful single peak is most likely to be hiding 11:09
- site_itch — the honest position is that hobby-level interpretation of a chromatogram is worth something but not much 11:14
- panel_before_after — update on the earlier thing i was wrong about tailing factor. it is usually the injection or the pH, not the column 13:32
thats co-elution
the honest position is that hobby-level interpretation of a chromatogram is worth something but not much
plus 0.98 is deamidation
ms/ms or it isnt identity
oxidation is about plus 15.99 Da and is usually methionine or tryptophan, ill dig out the number
retention time is a hint
within inter-lab range
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quick one whats the void volume peak, is that always solvent
tirzepatide is about 4813.5 Da, so 2+ is near 2407.8 and 3+ near 1605.5
if the lab reports area percent without stating the wavelength, the number is less useful than it looks
update on the earlier thing i was wrong about tailing factor. it is usually the injection or the pH, not the column
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whats a plus one dalton shift usually