baseline drift on a gradient is normal. baseline drift on an isocratic hold is your column
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Thread · #hplc-massspec · 2025-04-08
if the lab reports area percent without stating the
Spun off from a message in #hplc-massspec on 2025-04-08. 17 messages, 3 participants.
The message this came from
- cat_on_the_tray — if the lab reports area percent without stating the wavelength, the number is less useful than it looks 21:21
baseline drift on a gradient is normal. baseline drift on an isocratic hold is your column
need more sample
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within inter-lab range
baseline drift on a gradient is normal. baseline drift on an isocratic hold is your column
update from 11 months ago: switched to asking for the chromatogram rather than the number and it changed everything
plus 0.98 is deamidation
show me the spectrum
thats co-elution
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whats a plus one dalton shift usually
214nm sees the amide backbone so it sees everything. 280 only sees aromatics
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nobody runs enough blanks, including me
does retention time on its own tell you what the compound is
why does integration change the number so much
relative retention time against a standard is comparable between labs. absolute minutes are not
if somebody says they ran a mass spec and does not show you a spectrum, that is a claim, not a result
coming back after 13 months, what changed in how you read these