214nm sees the amide backbone so it sees everything. 280 only sees aromatics
Thread · #hplc-massspec · 2025-07-28
a warm transit can put a visible degradant peak
Spun off from a message in #hplc-massspec on 2025-07-28. 11 messages, 4 participants.
The message this came from
- vial_ledger — a warm transit can put a visible degradant peak on a chromatogram. that is what 24 days at ambient does 15:13
if the lab reports area percent without stating the wavelength, the number is less useful than it looks
baseline is drifting badly, is that the column or the mobile phase
nobody runs enough blanks, including me
if somebody says they ran a mass spec and does not show you a spectrum, that is a claim, not a result
need more sample
a certificate with a number and no chromatogram is asking you to trust the integration you cannot see
how would a truncation sequence show up
ms/ms sequencing is the only thing i would call identity. everything else is circumstantial
whats the void volume peak, is that always solvent
🙏5🎉14
plus 0.98 is deamidation