whats the void volume peak, is that always solvent
does retention time on its own tell you what
Spun off from a message in #hplc-massspec on 2026-03-14. 13 messages, 6 participants.
- protein_floor — does retention time on its own tell you what the compound is 16:55
coming back after 10 months, what changed in how you read these
the honest position is that hobby-level interpretation of a chromatogram is worth something but not much
baseline is drifting badly, is that the column or the mobile phase
is monoisotopic or average mass the one on most certificates
does retention time on its own tell you what the compound is
i have had a lot where the mass was exactly right and the purity was 96.8. wrong question, right answer
why does integration change the number so much
two labs a point or two apart on the same lot is normal and gets mistaken for one of them being wrong constantly
integration difference
oxidation is about plus 15.99 Da and is usually methionine or tryptophan
shallow gradients hide related substances behind the main peak. a steeper one separates and looks worse, honestly
thats co-elution