plus sixteen, is that always oxidation
Thread · #hplc-massspec · 2026-03-28
retention time is a hint, not a fingerprint. two
Spun off from a message in #hplc-massspec on 2026-03-28. 13 messages, 5 participants.
The message this came from
- dexa_or_bia — retention time is a hint, not a fingerprint. two different peptides can co-elute perfectly happily, ill dig out the number 21:41
a warm transit can put a visible degradant peak on a chromatogram. that is what 15 days at ambient does
🎉6
integration difference
show me the spectrum
ms/ms or it isnt identity
how much sample does a lab actually need for identity as well as purity
is there any point running a blank if the sample is the question
integration difference
ms/ms or it isnt identity
retention time is a hint, not a fingerprint. two different peptides can co-elute perfectly happily
😂2
is uv at 214 or 280 better for peptides
thats co-elution
❤️18
i was wrong about tailing factor. it is usually the injection or the pH, not the column