update on the earlier thing whats a plus one

semaglutide is about 4113.6 Da. [M+2H]2+ lands near 2057.8, [M+3H]3+ near 1372.2

relative retention time against a standard is comparable between labs. absolute minutes are not

i was wrong about tailing factor. it is usually the injection or the pH, not the column

ask for the trace

a certificate with a number and no chromatogram is asking you to trust the integration you cannot see

do you trust an area percent from a shallow gradient

the first peak is the solvent. it is always the solvent

a certificate with a number and no chromatogram is asking you to trust the integration you cannot see

a shoulder on a peak is information, and usually bad information