on the envelope thing at 214 you are detecting the peptide bond, so almost everything peptide shows up. at 280 only the aromatics do, ill dig out the number
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Spun off from a message in #hplc-massspec on 2026-05-13. 7 messages, 4 participants.
on the envelope thing at 214 you are detecting the peptide bond, so almost everything peptide shows up. at 280 only the aromatics do, ill dig out the number
pedantry about identity can you tell a dimer from the chromatogram alone
not always meaningful
on the envelope thing can you tell a deletion sequence from the mass alone
plus 0.98 deamidation
retention time is a hint, not a fingerprint. two different peptides can co-elute perfectly happily, ask for the blank
relative retention time against a standard is comparable between labs. absolute minutes are not, thats my reading anyway