can you tell a dimer from the chromatogram alone
Thread · #hplc-massspec · 2026-05-13
a warm transit can put a visible degradant peak
Spun off from a message in #hplc-massspec on 2026-05-13. 9 messages, 6 participants.
The message this came from
- still_here_2024 — a warm transit can put a visible degradant peak on a chromatogram. that is what 13 days at ambient does 19:39
if the number you are staring at is a third of what you expected, do the charge state arithmetic before you panic
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do you trust an area percent from a shallow gradient
update from 8 months ago: switched to asking for the chromatogram rather than the number and it changed everything
show me the spectrum
214nm sees the amide backbone so it sees everything. 280 only sees aromatics
how would a truncation sequence show up
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integration choices move the number by a few tenths easily. that is most of your inter-lab variance
a shoulder on a peak is information, and usually bad information