lyophilised powder is far more robust than people think. it is the solution that is fragile
[edited]#reconstitution 2024-07-19
Friday17 messages5 participantstimes are UTC
Highlights from this day
- third_shift — if the powder is on the stopper, tap it down before you add anything. it will still dissolve either way 14:34
- logrotate — my rule is one concentration for everything so i never have to think about it at 7am, not advice obviously 14:50
- VialBot — Recon calculator: 20mg in 5ml = 2.5mg/ml. 14:53
if the powder is on the stopper, tap it down before you add anything. it will still dissolve either way
does benzyl alcohol matter if im finishing the vial in one go
anyone else find the 30mg vials harder to get fully dissolved than the small ones
why does everyone say 2ml specifically and not 1ml
30mg in 3ml is 10mg/ml, so 5mg is 50 units. half the barrel, feels like a lot but its right
my rule is one concentration for everything so i never have to think about it at 7am, not advice obviously
the last few units are a real loss. i accept it rather than tilting the vial and drawing air
Recon calculator: 20mg in 5ml = 2.5mg/ml.
⚠️15
read it at eye level
10mg vial
+ 1.0ml -> 10.0 mg/ml 0.5mg = 5u 1mg = 10u
+ 2.0ml -> 5.0 mg/ml 0.5mg = 10u 1mg = 20u
+ 2.5ml -> 4.0 mg/ml 0.5mg = 12.5u (dont)
+ 5.0ml -> 2.0 mg/ml 0.5mg = 25u 1mg = 50uswirl it. do not shake it. the foam is denatured protein and you are not getting it back
you can absolutely just always use 2ml, and honestly most of us should
benzyl alcohol is what makes it bacteriostatic. it is about 0.9% typically, still working it out
5mg in 2ml gives 2.5mg/ml, 0.25mg is 10 units. good for a starting dose
10mg in 1ml is 10mg/ml, so 2.5mg is 25 units. thats the one i use
it foamed. is it ruined
how long after reconstituting before its actually dissolved