is there any reason not to just always use 2ml
#reconstitution 2024-08-20
Tuesday25 messages7 participantstimes are UTC
Highlights from this day
- ring_size_down — swirl it. do not shake it. the foam is denatured protein and you are not getting it back 19:15
- VialBot — Inter-lab diff for lot F-1330: 98.6% vs 99.4%. Within expected range. 19:41
- monoisotopic — you can absolutely just always use 2ml, and honestly most of us should 19:50
- private_script_pri — do not add more bac water to an already reconstituted vial, you have lost track of the concentration, thats just me 21:00
anyone else find the 40mg vials harder to get fully dissolved than the small ones
how much do i lose to dead space with a 29G pin
read it at eye level
bac water
took me a year to stop over-diluting. the measurement error at 4 units is worse than the convenience
one unit is 0.01ml
weigh it first
not sterile water
swirl it. do not shake it. the foam is denatured protein and you are not getting it back
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ok so i was wrong about this last year: it is fine to draw cold, it just stings more going in, not advice obviously
update on the earlier thing if the maths gives you 13.7 units, change the volume. do not try to hit 13.7
for the archive benzyl alcohol is what makes it bacteriostatic. it is about 0.9% typically
what do you do with the last 40 units when you cant draw them cleanly
i swirl for about thirty seconds and then leave it. never had one fail to dissolve
update from 18 months ago: switched to a single concentration and have not miscalculated since
clear or bin it
Inter-lab diff for lot F-1330: 98.6% vs 99.4%. Within expected range.
you can absolutely just always use 2ml, and honestly most of us should
janoshik-e-2205.pdf
2 pages · 248 KB · not retained in the public archive
label the vial
coa-b-0329.pdf
1 page · 307 KB · not retained in the public archive
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it foamed. is it ruined
the last few units are a real loss. i accept it rather than tilting the vial and drawing air
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my rule is one concentration for everything so i never have to think about it at 7am
it should be completely clear with no floaters. cloudy means stop
do not add more bac water to an already reconstituted vial, you have lost track of the concentration, thats just me
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