bac water or sterile water for something im going into weekly for 9 weeks
#reconstitution 2025-04-05
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is a slightly cloudy solution ever ok or is that always a bin it
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anyone worked out a recon volume that puts 2.4mg on a round unit mark
if you draw up with a bigger pin and inject with a fresh one you lose a bit more to dead space
while im here if the powder is on the stopper, tap it down before you add anything. it will still dissolve either way
can i add more bac water later to a vial ive already reconstituted
my vial pulled the water in on its own, is that the vacuum or did i do something
label the date too. you will not remember which vial is which by week 26
Purity check: no report on file for lot SG-1177. Nothing logged either way.
i have never once regretted using a rounder number and a slightly bigger volume
[edited]i use bac water even for a single-use vial because it stings less, which is not the reason it exists
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read it at eye level
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how do you all measure a half unit, or do you just not
genuine question coming back after 9 months, has the pinned recon volume advice changed
follow up if you cannot read the mark confidently, your volume is too small. redo it at a lower concentration
20mg in 2ml is 10mg/ml. 1mg is 10 units, 2mg is 20 units, easy
aim the diluent at the glass wall, not at the powder. it goes in gentler
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coming back to this gross powder mass is not net peptide. water and counterion are in that number, i have it written down somewhere
im getting bubbles in the barrel every single time, what am i doing wrong
nobody here should be telling you a dose. we can check your arithmetic, thats a different thing
yep 10 units
write the date on
swirl it. do not shake it. the foam is denatured protein and you are not getting it back
update from 6 months ago: switched to a single concentration and have not miscalculated since
10mg in 1ml is 10mg/ml, so 2.5mg is 25 units. thats the one i use
it should be completely clear with no floaters. cloudy means stop
5mg in 2ml gives 2.5mg/ml, 0.25mg is 10 units. good for a starting dose
check your maths
give it 8 minutes at room temperature before you decide it has not dissolved
lyophilised powder is far more robust than people think. it is the solution that is fragile, not advice obviously
a slightly collapsed cake is cosmetic. a puddle in the bottom is not
thats the vacuum
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what do you do with the last 8 units when you cant draw them cleanly
whats the smallest volume you would trust yourself to measure on a u100
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one unit is 0.01ml. 100 units is 1ml. everything else in this channel follows from that
the jewellery scale is a good habit but calibrate it, they drift badly
swirl dont shake
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is there any reason not to just always use 2ml
quick one bacteriostatic water for anything multi-use. sterile water has no preservative in it at all
clear or bin it
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a 3ml syringe for the diluent and a u100 for the dose is the setup most people here end up with
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i write the mg/ml on the vial with a sharpie the moment i reconstitute it
follow up sanity check my maths before i pin myself with something stupid
how much do i lose to dead space with a 29G pin
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you can absolutely just always use 2ml, and honestly most of us should
i was wrong about this last year: it is fine to draw cold, it just stings more going in