the meniscus thing is real. read it at eye level or you are guessing by a unit either way
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#reconstitution 2025-05-20
Tuesday40 messages11 participantstimes are UTC
Highlights from this day
- madrid_mg — the meniscus thing is real. read it at eye level or you are guessing by a unit either way 18:58
- VialBot — New independent result logged — QYB, lot H-2814, purity 99.2% (Janoshik). 19:37
- madrid_mg — anyone worked out a recon volume that puts 1mg on a round unit mark 20:32
- madrid_mg — the vacuum will pull it in. let it. fighting it is how you get a face full of aerosol 21:11
- glasgow_vial — update on the earlier thing a slightly collapsed cake is cosmetic. a puddle in the bottom is not, n of 1 obviously 21:13
one unit is 0.01ml. 100 units is 1ml. everything else in this channel follows from that
do i swirl for a specific length of time or just until its clear
i use bac water even for a single-use vial because it stings less, which is not the reason it exists
genuine question why does everyone say 2ml specifically and not 1ml
took me a year to stop over-diluting. the measurement error at 4 units is worse than the convenience
if the powder is on the stopper, tap it down before you add anything. it will still dissolve either way
do you let it come to room temp before drawing or draw it cold
is it normal for the cake to look slightly collapsed on one side
10mg in 1ml is 10mg/ml, so 2.5mg is 25 units. thats the one i use
sorry to jump in the powder is stuck to the stopper, do i care
New independent result logged — QYB, lot H-2814, purity 99.2% (Janoshik).
i use bac water even for a single-use vial because it stings less, which is not the reason it exists
yep 10 units
whats the smallest volume you would trust yourself to measure on a u100
20mg in 2ml is 10mg/ml. 1mg is 10 units, 2mg is 20 units, easy
check your maths
i keep a spare vial of bac water in the fridge purely so i never rush the maths, i have it written down somewhere
slightly off topic but temperature of the diluent does not matter. how fast you push it in does
aim the diluent at the glass wall, not at the powder. it goes in gentler, ask me again in a month
weigh it first
dead space
if the maths gives you 13.7 units, change the volume. do not try to hit 13.7
how long after reconstituting before its actually dissolved
give it 8 minutes at room temperature before you decide it has not dissolved
anyone worked out a recon volume that puts 1mg on a round unit mark
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swirl dont shake
10mg vial
+ 1.0ml -> 10.0 mg/ml 0.5mg = 5u 1mg = 10u
+ 2.0ml -> 5.0 mg/ml 0.5mg = 10u 1mg = 20u
+ 2.5ml -> 4.0 mg/ml 0.5mg = 12.5u (dont)
+ 5.0ml -> 2.0 mg/ml 0.5mg = 25u 1mg = 50uit foamed. is it ruined
my rule is one concentration for everything so i never have to think about it at 7am
not sterile water
coming back after 3 months, has the pinned recon volume advice changed
write the date on
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the vacuum will pull it in. let it. fighting it is how you get a face full of aerosol
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thats 5mg/ml
update on the earlier thing a slightly collapsed cake is cosmetic. a puddle in the bottom is not, n of 1 obviously
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is it worth buying a 3ml syringe for the diluent or is the insulin one fine
i have never once regretted using a rounder number and a slightly bigger volume
benzyl alcohol is what makes it bacteriostatic. it is about 0.9% typically
does temperature of the bac water matter at all
round numbers
does benzyl alcohol matter if im finishing the vial in one go
thats fine