bacteriostatic water for anything multi-use. sterile water has no preservative in it at all
#reconstitution 2025-07-17
Thursday36 messages9 participantstimes are UTC
Highlights from this day
- night_pin — my rule is one concentration for everything so i never have to think about it at 7am i use bac water even for a single-use vial because it stings less, which is not… 20:45
- bea_reconstitutes — the jewellery scale is a good habit but calibrate it, they drift badly 21:18
- rotterdam_recon — temperature of the diluent does not matter. how fast you push it in does if you cannot read the mark confidently, your volume is too small. redo it at a lower… 22:23
swirl it. do not shake it. the foam is denatured protein and you are not getting it back
not sterile water
thats fine
i write the mg/ml on the vial with a sharpie the moment i reconstitute it
aim the diluent at the glass wall, not at the powder. it goes in gentler
weigh it first
a 3ml syringe for the diluent and a u100 for the dose is the setup most people here end up with
you can absolutely just always use 2ml, and honestly most of us should
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how do you all measure a half unit, or do you just not
my rule is one concentration for everything so i never have to think about it at 7am
i use bac water even for a single-use vial because it stings less, which is not the reason it exists
the vacuum will pull it in. let it. fighting it is how you get a face full of aerosol
one unit is 0.01ml
whats the smallest volume you would trust yourself to measure on a u100
the last few units are a real loss. i accept it rather than tilting the vial and drawing air
10mg in 1ml is 10mg/ml, so 2.5mg is 25 units. thats the one i use
back_from_awayswirl it. do not shake it. the foam is denatured protein and you are not getting it back
anyone got a photo of what a properly dissolved vial looks like
read it at eye level
benzyl alcohol is what makes it bacteriostatic. it is about 0.9% typically, ask me again in a month
thats 5mg/ml
the jewellery scale is a good habit but calibrate it, they drift badly
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how long after reconstituting before its actually dissolved
update from 7 months ago: switched to a single concentration and have not miscalculated since
how much do i lose to dead space with a 29G pin
20mg in 2ml is 10mg/ml. 1mg is 10 units, 2mg is 20 units, easy
lyophilised powder is far more robust than people think. it is the solution that is fragile
yep 10 units
ok so one unit is 0.01ml. 100 units is 1ml. everything else in this channel follows from that
the powder is stuck to the stopper, do i care
check your maths
round numbers
temperature of the diluent does not matter. how fast you push it in does
if you cannot read the mark confidently, your volume is too small. redo it at a lower concentration
give it 5 minutes at room temperature before you decide it has not dissolved
clear or bin it
swirl dont shake