a slightly collapsed cake is cosmetic. a puddle in the bottom is not
#reconstitution 2025-08-03
Sunday47 messages11 participantstimes are UTC
Highlights from this day
- freeze_thaw — i swirl for about thirty seconds and then leave it. never had one fail to dissolve 22:33
- chlorhex — while im here i write the mg/ml on the vial with a sharpie the moment i reconstitute it 22:36
- snac_and_water — ok so anyone worked out a recon volume that puts 2.4mg on a round unit mark 22:53
- septum_sal — follow up is a slightly cloudy solution ever ok or is that always a bin it 23:02
- sharps_bin_sid — update from 12 months ago: switched to a single concentration and have not miscalculated since i was wrong about this last year: it is fine to draw cold, it just… 23:24
30mg in 3ml is 10mg/ml, so 5mg is 50 units. half the barrel, feels like a lot but its right
thats fine
does temperature of the bac water matter at all
yep 10 units
not sterile water
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thats 5mg/ml
swirl dont shake
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coming back after 4 months, has the pinned recon volume advice changed
if the maths gives you 13.7 units, change the volume. do not try to hit 13.7
i swirl for about thirty seconds and then leave it. never had one fail to dissolve
janoshik-e-2205.pdf
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nope, redo it
while im here i write the mg/ml on the vial with a sharpie the moment i reconstitute it
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my rule is one concentration for everything so i never have to think about it at 7am
one unit is 0.01ml. 100 units is 1ml. everything else in this channel follows from that
gross powder mass is not net peptide. water and counterion are in that number
i use bac water even for a single-use vial because it stings less, which is not the reason it exists, thats just me
if you cannot read the mark confidently, your volume is too small. redo it at a lower concentration
the vacuum will pull it in. let it. fighting it is how you get a face full of aerosol
how long after reconstituting before its actually dissolved
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why does everyone say 2ml specifically and not 1ml
i keep a spare vial of bac water in the fridge purely so i never rush the maths, thats just me
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ok so anyone worked out a recon volume that puts 2.4mg on a round unit mark
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bacteriostatic water for anything multi-use. sterile water has no preservative in it at all
lyophilised powder is far more robust than people think. it is the solution that is fragile
Testing queue: 2 submissions open, 50 awaiting dispatch.
coming back to this how do you all measure a half unit, or do you just not
follow up is a slightly cloudy solution ever ok or is that always a bin it
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pick the volume so your dose is a whole number of units. thats the entire trick
weigh it first
right so i was wrong about this last year: it is fine to draw cold, it just stings more going in
Archive lookup: septum_sal first appears in this channel on 2025-04-12.
do not add more bac water to an already reconstituted vial, you have lost track of the concentration
the last few units are a real loss. i accept it rather than tilting the vial and drawing air
while im here dead space in a standard insulin syringe hub is small but it is not nothing over a month
the meniscus thing is real. read it at eye level or you are guessing by a unit either way
label the date too. you will not remember which vial is which by week 21
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aim the diluent at the glass wall, not at the powder. it goes in gentler
update from 12 months ago: switched to a single concentration and have not miscalculated since
i was wrong about this last year: it is fine to draw cold, it just stings more going in
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slightly off topic but it should be completely clear with no floaters. cloudy means stop
foam is bad
the jewellery scale is a good habit but calibrate it, they drift badly
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one unit is 0.01ml
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the powder is stuck to the stopper, do i care
5mg in 2ml gives 2.5mg/ml, 0.25mg is 10 units. good for a starting dose
clear or bin it
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dead space