update on the earlier thing what do you do with the last 10 units when you cant draw them cleanly
#reconstitution 2025-10-17
Friday34 messages9 participantstimes are UTC
Highlights from this day
- vienna_vial — does benzyl alcohol matter if im finishing the vial in one go 19:22
- vienna_vial — i was wrong about this last year: it is fine to draw cold, it just stings more going in 19:41
- apob_over_ldl — do not add more bac water to an already reconstituted vial, you have lost track of the concentration 21:31
- drawup_dee — whats the smallest volume you would trust yourself to measure on a u100 the jewellery scale is a good habit but calibrate it, they drift badly 21:33
the vacuum will pull it in. let it. fighting it is how you get a face full of aerosol
bacteriostatic water for anything multi-use. sterile water has no preservative in it at all
i keep a spare vial of bac water in the fridge purely so i never rush the maths
5mg in 2ml gives 2.5mg/ml, 0.25mg is 10 units. good for a starting dose
you can absolutely just always use 2ml, and honestly most of us should, anyway
anyone got a photo of what a properly dissolved vial looks like
it foamed. is it ruined
my vial pulled the water in on its own, is that the vacuum or did i do something
read it at eye level
genuine question if the powder is on the stopper, tap it down before you add anything. it will still dissolve either way
swirl it. do not shake it. the foam is denatured protein and you are not getting it back
Purity check: no report on file for lot C-4402. Nothing logged either way.
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i have never once regretted using a rounder number and a slightly bigger volume
if the maths gives you 13.7 units, change the volume. do not try to hit 13.7, take that with a pinch of salt
if i add 1ml to a 20mg vial what concentration am i actually at
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gross powder mass is not net peptide. water and counterion are in that number
does benzyl alcohol matter if im finishing the vial in one go
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dead space in a standard insulin syringe hub is small but it is not nothing over a month
i was wrong about this last year: it is fine to draw cold, it just stings more going in
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weigh it first
U-100 syringe: 1 unit = 0.01 ml
10u = 0.10 ml
25u = 0.25 ml
50u = 0.50 ml
100u = 1.00 ml
mg drawn = ml drawn x mg/ml15mg in 3ml is 5mg/ml. same numbers as 10 in 2, just more of it
swirl dont shake
i use bac water even for a single-use vial because it stings less, which is not the reason it exists
if you cannot read the mark confidently, your volume is too small. redo it at a lower concentration
10mg in 2ml is 5mg/ml. so 0.5mg is 0.1ml which is 10 units on a u100
can i add more bac water later to a vial ive already reconstituted
weighing the vial before you reconstitute is the only way to catch an underfill
do not add more bac water to an already reconstituted vial, you have lost track of the concentration
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thats the vacuum
whats the smallest volume you would trust yourself to measure on a u100
the jewellery scale is a good habit but calibrate it, they drift badly
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why does everyone say 2ml specifically and not 1ml
label the vial
is a slightly cloudy solution ever ok or is that always a bin it