do not add more bac water to an already reconstituted vial, you have lost track of the concentration
#reconstitution 2025-10-24
Friday19 messages7 participantstimes are UTC
Highlights from this day
New independent result logged — TFC, lot B-0114, purity 98.6% (VendorInvestigate).
if the powder is on the stopper, tap it down before you add anything. it will still dissolve either way
a 3ml syringe for the diluent and a u100 for the dose is the setup most people here end up with
swirl it. do not shake it. the foam is denatured protein and you are not getting it back
5mg in 2ml gives 2.5mg/ml, 0.25mg is 10 units. good for a starting dose
anyone else find the 30mg vials harder to get fully dissolved than the small ones
*VendorInvestigate not the other one
sorry to jump in my 10mg vial weighed 11.4g on the jewellery scale, is that wrong
lyophilised powder is far more robust than people think. it is the solution that is fragile
my rule is one concentration for everything so i never have to think about it at 7am, ill dig out the number
not sterile water
i keep a spare vial of bac water in the fridge purely so i never rush the maths
one unit is 0.01ml. 100 units is 1ml. everything else in this channel follows from that
[edited]im getting bubbles in the barrel every single time, what am i doing wrong
pick the volume so your dose is a whole number of units. thats the entire trick
the powder is stuck to the stopper, do i care
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the last few units are a real loss. i accept it rather than tilting the vial and drawing air
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yep 10 units
if you cannot read the mark confidently, your volume is too small. redo it at a lower concentration
aim the diluent at the glass wall, not at the powder. it goes in gentler