yep 10 units
#reconstitution 2026-01-10
Saturday61 messages14 participantstimes are UTC
Highlights from this day
- VialBot — Inter-lab diff for lot H-2814: 99.4% vs 99.2%. Within expected range. 16:59
- kev_measures — while im here it foamed. is it ruined 17:18
- triumph_watch — is the meniscus reading actually a real source of error or am i overthinking 17:54
- swirl_not_shake — is a slightly cloudy solution ever ok or is that always a bin it 19:23
does it matter if i inject the bac water straight at the powder
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do i swirl for a specific length of time or just until its clear
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right so label the date too. you will not remember which vial is which by week 18
my rule is one concentration for everything so i never have to think about it at 7am
my vial pulled the water in on its own, is that the vacuum or did i do something
20mg in 2ml is 10mg/ml. 1mg is 10 units, 2mg is 20 units, easy, thats just me
im getting bubbles in the barrel every single time, what am i doing wrong
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foam is bad
[edited]Inter-lab diff for lot H-2814: 99.4% vs 99.2%. Within expected range.
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if the maths gives you 13.7 units, change the volume. do not try to hit 13.7
a 3ml syringe for the diluent and a u100 for the dose is the setup most people here end up with
took me a year to stop over-diluting. the measurement error at 4 units is worse than the convenience
how do you all measure a half unit, or do you just not
anyone else find the 10mg vials harder to get fully dissolved than the small ones
nobody here should be telling you a dose. we can check your arithmetic, thats a different thing
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while im here it foamed. is it ruined
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bac water
5mg in 2ml gives 2.5mg/ml, 0.25mg is 10 units. good for a starting dose
thats the vacuum
15mg in 3ml is 5mg/ml. same numbers as 10 in 2, just more of it
10mg in 1ml is 10mg/ml, so 2.5mg is 25 units. thats the one i use
sorry to jump in one unit is 0.01ml. 100 units is 1ml. everything else in this channel follows from that
30mg in 3ml is 10mg/ml, so 5mg is 50 units. half the barrel, feels like a lot but its right
weigh it first
the meniscus thing is real. read it at eye level or you are guessing by a unit either way
read it at eye level
pick the volume so your dose is a whole number of units. thats the entire trick
aim the diluent at the glass wall, not at the powder. it goes in gentler, still working it out
is the meniscus reading actually a real source of error or am i overthinking
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the powder is stuck to the stopper, do i care
round numbers
coming back after 16 months, has the pinned recon volume advice changed
what do you do with the last 20 units when you cant draw them cleanly
write the date on
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i swirl for about thirty seconds and then leave it. never had one fail to dissolve
anyone got a photo of what a properly dissolved vial looks like
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unrelated but is it normal for the cake to look slightly collapsed on one side
nope, redo it
does benzyl alcohol matter if im finishing the vial in one go
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sanity check my maths before i pin myself with something stupid
bac water or sterile water for something im going into weekly for 5 weeks
update from 5 months ago: switched to a single concentration and have not miscalculated since
thats fine
do you let it come to room temp before drawing or draw it cold
10mg vial
+ 1.0ml -> 10.0 mg/ml 0.5mg = 5u 1mg = 10u
+ 2.0ml -> 5.0 mg/ml 0.5mg = 10u 1mg = 20u
+ 2.5ml -> 4.0 mg/ml 0.5mg = 12.5u (dont)
+ 5.0ml -> 2.0 mg/ml 0.5mg = 25u 1mg = 50uis a slightly cloudy solution ever ok or is that always a bin it
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not sterile water
weighing the vial before you reconstitute is the only way to catch an underfill
you can absolutely just always use 2ml, and honestly most of us should, your mileage will differ
a slightly collapsed cake is cosmetic. a puddle in the bottom is not
check your maths
temperature of the diluent does not matter. how fast you push it in does
20mg in 2ml is 10mg/ml. 1mg is 10 units, 2mg is 20 units, easy
clear or bin it
do not add more bac water to an already reconstituted vial, you have lost track of the concentration
can i add more bac water later to a vial ive already reconstituted
why does everyone say 2ml specifically and not 1ml
i swirl for about thirty seconds and then leave it. never had one fail to dissolve
do you draw up with the same pin you inject with
label the vial
the last few units are a real loss. i accept it rather than tilting the vial and drawing air
if you draw up with a bigger pin and inject with a fresh one you lose a bit more to dead space