lyophilised powder is far more robust than people think. it is the solution that is fragile
#reconstitution 2026-02-05
Thursday55 messages9 participantstimes are UTC
Highlights from this day
- sol_sends — i swirl for about thirty seconds and then leave it. never had one fail to dissolve 20:40
- hk_hplc — if the maths gives you 13.7 units, change the volume. do not try to hit 13.7 21:27
- LC_MS_Lena — update from 1 months ago: switched to a single concentration and have not miscalculated since 21:47
- LC_MS_Lena — i have never once regretted using a rounder number and a slightly bigger volume 21:48
give it 9 minutes at room temperature before you decide it has not dissolved
a slightly collapsed cake is cosmetic. a puddle in the bottom is not
yep 10 units
im getting bubbles in the barrel every single time, what am i doing wrong
coming back to this is there any reason not to just always use 2ml
does benzyl alcohol matter if im finishing the vial in one go
ok so 15mg in 3ml is 5mg/ml. same numbers as 10 in 2, just more of it
round numbers
weigh it first
write the date on
clear or bin it
dead space
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do i swirl for a specific length of time or just until its clear
label the vial
how do you all measure a half unit, or do you just not
for the archive aim the diluent at the glass wall, not at the powder. it goes in gentler
do not add more bac water to an already reconstituted vial, you have lost track of the concentration, for what its worth
you can absolutely just always use 2ml, and honestly most of us should, happy to be corrected
nope, redo it
bac water or sterile water for something im going into weekly for 3 weeks
bacteriostatic water for anything multi-use. sterile water has no preservative in it at all
bac water
what do you do with the last 40 units when you cant draw them cleanly
temperature of the diluent does not matter. how fast you push it in does
does temperature of the bac water matter at all
thats fine
slightly off topic but i use bac water even for a single-use vial because it stings less, which is not the reason it exists
benzyl alcohol is what makes it bacteriostatic. it is about 0.9% typically
for the archive 20mg in 2ml is 10mg/ml. 1mg is 10 units, 2mg is 20 units, easy
not sterile water
whats the smallest volume you would trust yourself to measure on a u100
took me a year to stop over-diluting. the measurement error at 4 units is worse than the convenience
*PeptideMeter not the other one
i swirl for about thirty seconds and then leave it. never had one fail to dissolve
U-100 syringe: 1 unit = 0.01 ml
10u = 0.10 ml
25u = 0.25 ml
50u = 0.50 ml
100u = 1.00 ml
mg drawn = ml drawn x mg/mli write the mg/ml on the vial with a sharpie the moment i reconstitute it
sorry to jump in it should be completely clear with no floaters. cloudy means stop
does it matter if i inject the bac water straight at the powder
10mg in 2ml is 5mg/ml. so 0.5mg is 0.1ml which is 10 units on a u100
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foam is bad
i keep a spare vial of bac water in the fridge purely so i never rush the maths
if the maths gives you 13.7 units, change the volume. do not try to hit 13.7
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i was wrong about this last year: it is fine to draw cold, it just stings more going in
dead space in a standard insulin syringe hub is small but it is not nothing over a month
slightly off topic but the jewellery scale is a good habit but calibrate it, they drift badly
took me a year to stop over-diluting. the measurement error at 4 units is worse than the convenience
weighing the vial before you reconstitute is the only way to catch an underfill
update from 1 months ago: switched to a single concentration and have not miscalculated since
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i have never once regretted using a rounder number and a slightly bigger volume
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one unit is 0.01ml. 100 units is 1ml. everything else in this channel follows from that
10mg in 2ml is 5mg/ml. so 0.5mg is 0.1ml which is 10 units on a u100
the powder is stuck to the stopper, do i care
my rule is one concentration for everything so i never have to think about it at 7am, thats just me
too dilute imo
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my 10mg vial weighed 11.4g on the jewellery scale, is that wrong
my vial pulled the water in on its own, is that the vacuum or did i do something
update on the earlier thing if the powder is on the stopper, tap it down before you add anything. it will still dissolve either way, for what its worth
is it normal for the cake to look slightly collapsed on one side
anyone else find the 2mg vials harder to get fully dissolved than the small ones