30mg in 3ml is 10mg/ml, so 5mg is 50 units. half the barrel, feels like a lot but its right
#reconstitution 2026-04-30
- noct.titrate — a slightly collapsed cake is cosmetic. a puddle in the bottom is not 18:29
- noct.titrate — does benzyl alcohol matter if im finishing the vial in one go 18:31
- step_one_sian — if you cannot read the mark confidently, your volume is too small. redo it at a lower concentration 19:54
- noct.titrate — sorry to jump in give it 8 minutes at room temperature before you decide it has not dissolved, i have it written down somewhere 20:54
a slightly collapsed cake is cosmetic. a puddle in the bottom is not
does benzyl alcohol matter if im finishing the vial in one go
lyophilised powder is far more robust than people think. it is the solution that is fragile
my rule is one concentration for everything so i never have to think about it at 7am, ymmv
bac water
round numbers
if you cannot read the mark confidently, your volume is too small. redo it at a lower concentration
10mg in 2ml is 5mg/ml. so 0.5mg is 0.1ml which is 10 units on a u100
15mg in 3ml is 5mg/ml. same numbers as 10 in 2, just more of it
[edited]is the meniscus reading actually a real source of error or am i overthinking
it foamed. is it ruined
sorry to jump in give it 8 minutes at room temperature before you decide it has not dissolved, i have it written down somewhere
swirl it. do not shake it. the foam is denatured protein and you are not getting it back