swirl it. do not shake it. the foam is denatured protein and you are not getting it back
#reconstitution 2026-05-28
Thursday57 messages14 participantstimes are UTC
Highlights from this day
- taper_tess — 5mg in 2ml gives 2.5mg/ml, 0.25mg is 10 units. good for a starting dose 21:30
- lyophile_liv — temperature of the diluent does not matter. how fast you push it in does, i have it written down somewhere 21:34
- ivy_injects — i use bac water even for a single-use vial because it stings less, which is not the reason it exists 5mg in 2ml gives 2.5mg/ml, 0.25mg is 10 units. good for a… 22:05
- wrong_network — can i add more bac water later to a vial ive already reconstituted 23:11
5mg in 2ml gives 2.5mg/ml, 0.25mg is 10 units. good for a starting dose
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one unit is 0.01ml
temperature of the diluent does not matter. how fast you push it in does, i have it written down somewhere
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benzyl alcohol is what makes it bacteriostatic. it is about 0.9% typically
i was wrong about this last year: it is fine to draw cold, it just stings more going in, anyway
[edited]is it worth buying a 3ml syringe for the diluent or is the insulin one fine
if the maths gives you 13.7 units, change the volume. do not try to hit 13.7
my 10mg vial weighed 11.4g on the jewellery scale, is that wrong
does benzyl alcohol matter if im finishing the vial in one go
weigh it first
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thats fine
i keep a spare vial of bac water in the fridge purely so i never rush the maths, i have it written down somewhere
anyone else find the 10mg vials harder to get fully dissolved than the small ones
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Verification log updated: QST — evidence added, status unchanged.
aim the diluent at the glass wall, not at the powder. it goes in gentler
if the powder is on the stopper, tap it down before you add anything. it will still dissolve either way
check your maths
update on the earlier thing coming back after 15 months, has the pinned recon volume advice changed
i use bac water even for a single-use vial because it stings less, which is not the reason it exists
5mg in 2ml gives 2.5mg/ml, 0.25mg is 10 units. good for a starting dose
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label the vial
U-100 syringe: 1 unit = 0.01 ml
10u = 0.10 ml
25u = 0.25 ml
50u = 0.50 ml
100u = 1.00 ml
mg drawn = ml drawn x mg/ml10mg in 1ml is 10mg/ml, so 2.5mg is 25 units. thats the one i use
took me a year to stop over-diluting. the measurement error at 4 units is worse than the convenience
if i add 0.5ml to a 30mg vial what concentration am i actually at
how do you all measure a half unit, or do you just not
give it 8 minutes at room temperature before you decide it has not dissolved
im getting bubbles in the barrel every single time, what am i doing wrong
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my rule is one concentration for everything so i never have to think about it at 7am
weighing the vial before you reconstitute is the only way to catch an underfill
gross powder mass is not net peptide. water and counterion are in that number
thats 5mg/ml
coming back to this a 3ml syringe for the diluent and a u100 for the dose is the setup most people here end up with, we shall see
if you draw up with a bigger pin and inject with a fresh one you lose a bit more to dead space
Verification log updated: QST — evidence added, status unchanged.
sorry to jump in label the date too. you will not remember which vial is which by week 22, from memory
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nobody here should be telling you a dose. we can check your arithmetic, thats a different thing
took me a year to stop over-diluting. the measurement error at 4 units is worse than the convenience
10mg vial
+ 1.0ml -> 10.0 mg/ml 0.5mg = 5u 1mg = 10u
+ 2.0ml -> 5.0 mg/ml 0.5mg = 10u 1mg = 20u
+ 2.5ml -> 4.0 mg/ml 0.5mg = 12.5u (dont)
+ 5.0ml -> 2.0 mg/ml 0.5mg = 25u 1mg = 50uunrelated but lyophilised powder is far more robust than people think. it is the solution that is fragile
is the meniscus reading actually a real source of error or am i overthinking
too dilute imo
clear or bin it
the meniscus thing is real. read it at eye level or you are guessing by a unit either way
thats the vacuum
i have never once regretted using a rounder number and a slightly bigger volume
[edited]read it at eye level
round numbers
what do you do with the last 20 units when you cant draw them cleanly
do you let it come to room temp before drawing or draw it cold
a slightly collapsed cake is cosmetic. a puddle in the bottom is not
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do not add more bac water to an already reconstituted vial, you have lost track of the concentration, i think
benzyl alcohol is what makes it bacteriostatic. it is about 0.9% typically
bac water or sterile water for something im going into weekly for 3 weeks
fridge-thermometer.png
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can i add more bac water later to a vial ive already reconstituted
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is it normal for the cake to look slightly collapsed on one side
one unit is 0.01ml. 100 units is 1ml. everything else in this channel follows from that
weigh it first
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you can absolutely just always use 2ml, and honestly most of us should
my vial pulled the water in on its own, is that the vacuum or did i do something