i use bac water even for a single-use vial because it stings less, which is not the reason it exists
#reconstitution 2026-06-21
Sunday66 messages15 participantstimes are UTC
Highlights from this day
- batch_bandit — sanity check my maths before i pin myself with something stupid 18:07
- birch_bloods — weighing the vial before you reconstitute is the only way to catch an underfill 19:06
- bea_reconstitutes — update on the earlier thing how much do i lose to dead space with a 31G pin 19:41
- unsolicited_dm — give it 6 minutes at room temperature before you decide it has not dissolved, we shall see pick the volume so your dose is a whole number of units. thats the entire… 20:18
- yyz_vial — does it matter if i inject the bac water straight at the powder 21:08
nope, redo it
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follow up i have never once regretted using a rounder number and a slightly bigger volume
im getting bubbles in the barrel every single time, what am i doing wrong
i was wrong about this last year: it is fine to draw cold, it just stings more going in
do you draw up with the same pin you inject with
label the vial
10mg in 2ml is 5mg/ml. so 0.5mg is 0.1ml which is 10 units on a u100
quick one 30mg in 3ml is 10mg/ml, so 5mg is 50 units. half the barrel, feels like a lot but its right
for the archive label the date too. you will not remember which vial is which by week 6, n of 1 obviously
the last few units are a real loss. i accept it rather than tilting the vial and drawing air
U-100 syringe: 1 unit = 0.01 ml
10u = 0.10 ml
25u = 0.25 ml
50u = 0.50 ml
100u = 1.00 ml
mg drawn = ml drawn x mg/mlnobody here should be telling you a dose. we can check your arithmetic, thats a different thing
sanity check my maths before i pin myself with something stupid
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dead space in a standard insulin syringe hub is small but it is not nothing over a month, i think
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aim the diluent at the glass wall, not at the powder. it goes in gentler
weigh it first
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swirl dont shake
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you can absolutely just always use 2ml, and honestly most of us should
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dead space
it foamed. is it ruined
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how long after reconstituting before its actually dissolved
gross powder mass is not net peptide. water and counterion are in that number
quick one one unit is 0.01ml. 100 units is 1ml. everything else in this channel follows from that
is a slightly cloudy solution ever ok or is that always a bin it
it should be completely clear with no floaters. cloudy means stop
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ok so what do you do with the last 25 units when you cant draw them cleanly
one unit is 0.01ml. 100 units is 1ml. everything else in this channel follows from that
one unit is 0.01ml
slightly off topic but if the maths gives you 13.7 units, change the volume. do not try to hit 13.7
pick the volume so your dose is a whole number of units. thats the entire trick
weighing the vial before you reconstitute is the only way to catch an underfill
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thats 5mg/ml
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5mg in 2ml gives 2.5mg/ml, 0.25mg is 10 units. good for a starting dose, from memory
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thats the vacuum
foam is bad
ok so is it worth buying a 3ml syringe for the diluent or is the insulin one fine
update on the earlier thing how much do i lose to dead space with a 31G pin
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*PeptideMeter not the other one
round numbers
bac water or sterile water for something im going into weekly for 3 weeks
do not add more bac water to an already reconstituted vial, you have lost track of the concentration
while im here does temperature of the bac water matter at all
is the meniscus reading actually a real source of error or am i overthinking
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if i add 0.5ml to a 2mg vial what concentration am i actually at
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lyophilised powder is far more robust than people think. it is the solution that is fragile
U-100 syringe: 1 unit = 0.01 ml
10u = 0.10 ml
25u = 0.25 ml
50u = 0.50 ml
100u = 1.00 ml
mg drawn = ml drawn x mg/mlfollow up i keep a spare vial of bac water in the fridge purely so i never rush the maths
give it 6 minutes at room temperature before you decide it has not dissolved, we shall see
pick the volume so your dose is a whole number of units. thats the entire trick
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clear or bin it
20mg in 2ml is 10mg/ml. 1mg is 10 units, 2mg is 20 units, easy
gross powder mass is not net peptide. water and counterion are in that number
anyone got a photo of what a properly dissolved vial looks like
yep 10 units
check your maths
i write the mg/ml on the vial with a sharpie the moment i reconstitute it
bac water
how do you all measure a half unit, or do you just not
thats fine
can i add more bac water later to a vial ive already reconstituted
bacteriostatic water for anything multi-use. sterile water has no preservative in it at all
benzyl alcohol is what makes it bacteriostatic. it is about 0.9% typically
does it matter if i inject the bac water straight at the powder
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i swirl for about thirty seconds and then leave it. never had one fail to dissolve
the powder is stuck to the stopper, do i care
update from 2 months ago: switched to a single concentration and have not miscalculated since
Recon calculator: 40mg in 2ml = 5mg/ml.
a 3ml syringe for the diluent and a u100 for the dose is the setup most people here end up with
Purity check: no report on file for lot B-0114. Nothing logged either way.