follow up oxidation is about plus 15.99 Da and is usually methionine or tryptophan
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#hplc-massspec 2025-06-21
Saturday12 messages4 participantstimes are UTC
Highlights from this day
- third_shift — follow up oxidation is about plus 15.99 Da and is usually methionine or tryptophan 13:52
- HPLC_Hank — baseline is drifting badly, is that the column or the mobile phase 14:33
- tail_factor — the honest position is that hobby-level interpretation of a chromatogram is worth something but not much deamidation is about plus 0.98 Da. it is not a rounding… 15:11
a warm transit can put a visible degradant peak on a chromatogram. that is what 12 days at ambient does, for what its worth
ms/ms or it isnt identity
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while im here plus sixteen, is that always oxidation
within inter-lab range
is monoisotopic or average mass the one on most certificates
monoisotopic and average diverge more the bigger the molecule. say which one you are quoting
co-elution is the failure mode that a beautiful single peak is most likely to be hiding, not advice obviously
i asked Medutest for the raw trace and they sent it. that is the right answer to that question
baseline is drifting badly, is that the column or the mobile phase
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i have had a lot where the mass was exactly right and the purity was 96.8. wrong question, right answer
the honest position is that hobby-level interpretation of a chromatogram is worth something but not much
deamidation is about plus 0.98 Da. it is not a rounding error, it is a degradant
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