plus 0.98 is deamidation
#hplc-massspec 2025-09-01
Monday10 messages3 participantstimes are UTC
Highlights from this day
- ms_ms_mira — integration choices move the number by a few tenths easily. that is most of your inter-lab variance ms/ms sequencing is the only thing i would call identity.… 19:53
- endotoxin_ed — relative retention time against a standard is comparable between labs. absolute minutes are not 20:43
- bea_reconstitutes — plus sixteen, is that always oxidation 22:41
my peak has a shoulder on it, what does that usually mean
integration choices move the number by a few tenths easily. that is most of your inter-lab variance
ms/ms sequencing is the only thing i would call identity. everything else is circumstantial
how do you spot a deletion in a sequence from mass alone
thats a charge state
how do you tell co-elution from a clean single peak
relative retention time against a standard is comparable between labs. absolute minutes are not
deamidation is about plus 0.98 Da. it is not a rounding error, it is a degradant
do the arithmetic
plus sixteen, is that always oxidation
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