i was wrong about tailing factor. it is usually the injection or the pH, not the column, ill dig out the number
#hplc-massspec 2025-08-27
Wednesday14 messages3 participantstimes are UTC
Highlights from this day
- logrotate — i was wrong about tailing factor. it is usually the injection or the pH, not the column, ill dig out the number 17:54
- mhra_unlicensed — my peak has a shoulder on it, what does that usually mean 17:56
- mhra_unlicensed — unrelated but baseline drift on a gradient is normal. baseline drift on an isocratic hold is your column 18:35
- mhra_unlicensed — i asked Medutest for the raw trace and they sent it. that is the right answer to that question 18:36
- VialBot — Recon calculator: 15mg in 5ml = 2mg/ml. 18:40
my peak has a shoulder on it, what does that usually mean
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how do you tell co-elution from a clean single peak
a warm transit can put a visible degradant peak on a chromatogram. that is what 18 days at ambient does
*that should say weekly
retention time is a hint
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co-elution is the failure mode that a beautiful single peak is most likely to be hiding
ms/ms or it isnt identity
need more sample
unrelated but baseline drift on a gradient is normal. baseline drift on an isocratic hold is your column
i asked Medutest for the raw trace and they sent it. that is the right answer to that question
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semaglutide is about 4113.6 Da. [M+2H]2+ lands near 2057.8, [M+3H]3+ near 1372.2, still working it out
Recon calculator: 15mg in 5ml = 2mg/ml.