baseline drift on a gradient is normal. baseline drift on an isocratic hold is your column
#hplc-massspec 2025-11-02
Sunday16 messages4 participantstimes are UTC
Highlights from this day
- HPLC_Hank — update from 8 months ago: switched to asking for the chromatogram rather than the number and it changed everything 14:28
- vialkeeper — slightly off topic but a shoulder on a peak is information, and usually bad information, i think 14:44
- HPLC_Hank — ms/ms sequencing is the only thing i would call identity. everything else is circumstantial, happy to be corrected 14:45
- VialBot — Verification log updated: GL Biochem — evidence added, status unchanged. 15:56
- vialkeeper — nobody runs enough blanks, including me 17:59
update from 8 months ago: switched to asking for the chromatogram rather than the number and it changed everything
ask for the trace
slightly off topic but a shoulder on a peak is information, and usually bad information, i think
medutest-report-kp-0925.pdf
3 pages · 659 KB · not retained in the public archive
ms/ms sequencing is the only thing i would call identity. everything else is circumstantial, happy to be corrected
relative retention time against a standard is comparable between labs. absolute minutes are not
Verification log updated: GL Biochem — evidence added, status unchanged.
plus 0.98 is deamidation
show me the spectrum
need more sample
within inter-lab range
whats a plus one dalton shift usually
whats a normal gradient for a peptide this size
coming back after 6 months, what changed in how you read these
nobody runs enough blanks, including me
👍11😂14📈3
run a blank