integration difference
#hplc-massspec 2026-04-02
Thursday17 messages3 participantstimes are UTC
Highlights from this day
- taper_tess — a warm transit can put a visible degradant peak on a chromatogram. that is what 7 days at ambient does, from memory 16:44
- careful_claims — nobody runs enough blanks, including me monoisotopic and average diverge more the bigger the molecule. say which one you are quoting 17:39
- careful_claims — baseline is drifting badly, is that the column or the mobile phase 17:41
need more sample
a certificate with a number and no chromatogram is asking you to trust the integration you cannot see
slightly off topic but i was wrong about tailing factor. it is usually the injection or the pH, not the column
*PeptideMeter not the other one
a warm transit can put a visible degradant peak on a chromatogram. that is what 7 days at ambient does, from memory
📈8⚠️3
thats co-elution
i asked VendorInvestigate for the raw trace and they sent it. that is the right answer to that question
run a blank
shallow gradient
is a tailing factor of anything specific a problem
⚠️2
baseline drift on a gradient is normal. baseline drift on an isocratic hold is your column
nobody runs enough blanks, including me
monoisotopic and average diverge more the bigger the molecule. say which one you are quoting
thats the solvent peak
baseline is drifting badly, is that the column or the mobile phase
🔥6
do the arithmetic
show me the spectrum
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