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Thread · #hplc-massspec · 2025-12-03

baseline is drifting badly, is that the column or

Spun off from a message in #hplc-massspec on 2025-12-03. 7 messages, 4 participants.

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  • drawup_dee — baseline is drifting badly, is that the column or the mobile phase 22:55
CO

truncations show as a mass deficit matching one or more residues. you need the sequence to say which

AP

214nm sees the amide backbone so it sees everything. 280 only sees aromatics

⚠️11🤝14
CO

if the lab reports area percent without stating the wavelength, the number is less useful than it looks