vialroom

#reconstitution 2024-10-25

Friday31 messages8 participantstimes are UTC
Highlights from this day
  • residual_solvent — the vacuum will pull it in. let it. fighting it is how you get a face full of aerosol 14:59
  • dublin_dose — update on the earlier thing bacteriostatic water for anything multi-use. sterile water has no preservative in it at all 16:04
  • cagri_curious — it should be completely clear with no floaters. cloudy means stop 16:07
RS

do not add more bac water to an already reconstituted vial, you have lost track of the concentration

RS

took me a year to stop over-diluting. the measurement error at 4 units is worse than the convenience, someone check my working

SS

i swirl for about thirty seconds and then leave it. never had one fail to dissolve

SS

if you draw up with a bigger pin and inject with a fresh one you lose a bit more to dead space, thats just me

RS

one unit is 0.01ml. 100 units is 1ml. everything else in this channel follows from that

the last few units are a real loss. i accept it rather than tilting the vial and drawing air

WZ

update from 10 months ago: switched to a single concentration and have not miscalculated since, anyway

LL

update on the earlier thing you can absolutely just always use 2ml, and honestly most of us should, i could be wrong

AP

temperature of the diluent does not matter. how fast you push it in does

LL

pick the volume so your dose is a whole number of units. thats the entire trick

AP

slightly off topic but if the maths gives you 13.7 units, change the volume. do not try to hit 13.7

WZ

sorry to jump in do you let it come to room temp before drawing or draw it cold
lyophilised powder is far more robust than people think. it is the solution that is fragile

CH

gross powder mass is not net peptide. water and counterion are in that number, for what its worth

RS

the vacuum will pull it in. let it. fighting it is how you get a face full of aerosol

📈17🤝1
CH

benzyl alcohol is what makes it bacteriostatic. it is about 0.9% typically

i have never once regretted using a rounder number and a slightly bigger volume

i keep a spare vial of bac water in the fridge purely so i never rush the maths

CC

30mg in 3ml is 10mg/ml, so 5mg is 50 units. half the barrel, feels like a lot but its right

15:19chlorhex pinned a message to this channel
DD

update on the earlier thing bacteriostatic water for anything multi-use. sterile water has no preservative in it at all

👀4😂17
CC

it should be completely clear with no floaters. cloudy means stop

💀138⚠️3
LL

15mg in 3ml is 5mg/ml. same numbers as 10 in 2, just more of it
one unit is 0.01ml. 100 units is 1ml. everything else in this channel follows from that

anyone worked out a recon volume that puts 2.4mg on a round unit mark

nope, redo it

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10mg in 2ml is 5mg/ml. so 0.5mg is 0.1ml which is 10 units on a u100
if you draw up with a bigger pin and inject with a fresh one you lose a bit more to dead space

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