vialroom

#reconstitution 2024-10-29

Tuesday31 messages10 participantstimes are UTC
Highlights from this day
  • verify_or_dont — if you cannot read the mark confidently, your volume is too small. redo it at a lower concentration 18:09
  • lyophile_liv — how do you all measure a half unit, or do you just not 18:16
  • VialBot — New independent result logged — Homopeptide, lot SG-1177, purity 98.6% (PeptideMeter). 19:07
VO

if you cannot read the mark confidently, your volume is too small. redo it at a lower concentration

🎉4🔥11
SB

if the maths gives you 13.7 units, change the volume. do not try to hit 13.7

how do you all measure a half unit, or do you just not

👀185

how long after reconstituting before its actually dissolved

VO

while im here i swirl for about thirty seconds and then leave it. never had one fail to dissolve, thats one data point

⚠️3
LL

does it matter if i inject the bac water straight at the powder

[edited]

took me a year to stop over-diluting. the measurement error at 4 units is worse than the convenience

U1

im getting bubbles in the barrel every single time, what am i doing wrong

🎉1❤️10👍1
VB

New independent result logged — Homopeptide, lot SG-1177, purity 98.6% (PeptideMeter).

U1

label the date too. you will not remember which vial is which by week 24

not sterile water

U1

10mg in 2ml is 5mg/ml. so 0.5mg is 0.1ml which is 10 units on a u100, still working it out

RT

weighing the vial before you reconstitute is the only way to catch an underfill

EM

update on the earlier thing coming back after 23 months, has the pinned recon volume advice changed

[edited]
RT

i keep a spare vial of bac water in the fridge purely so i never rush the maths

i use bac water even for a single-use vial because it stings less, which is not the reason it exists, someone check my working