vialroom

#reconstitution 2025-03-05

Wednesday23 messages6 participantstimes are UTC
Highlights from this day
  • blind_submit — 5mg in 2ml gives 2.5mg/ml, 0.25mg is 10 units. good for a starting dose 18:13
  • meal_prep_mick — coming back after 22 months, has the pinned recon volume advice changed 18:35
  • VialBot — New independent result logged — JEEP, lot E-2205, purity 99% (Janoshik). 19:23
  • two_eight_c — my vial pulled the water in on its own, is that the vacuum or did i do something 20:03
  • two_eight_c — swirl it. do not shake it. the foam is denatured protein and you are not getting it back 20:40
BS

5mg in 2ml gives 2.5mg/ml, 0.25mg is 10 units. good for a starting dose

📉3

30mg in 3ml is 10mg/ml, so 5mg is 50 units. half the barrel, feels like a lot but its right

anyone got a photo of what a properly dissolved vial looks like

MP

coming back after 22 months, has the pinned recon volume advice changed

🎉5
TE

if you cannot read the mark confidently, your volume is too small. redo it at a lower concentration

pick the volume so your dose is a whole number of units. thats the entire trick

TE

it should be completely clear with no floaters. cloudy means stop

EM

you can absolutely just always use 2ml, and honestly most of us should

VB

New independent result logged — JEEP, lot E-2205, purity 99% (Janoshik).

🧊3👍5
TE

a slightly collapsed cake is cosmetic. a puddle in the bottom is not

update from 5 months ago: switched to a single concentration and have not miscalculated since

EM

one unit is 0.01ml. 100 units is 1ml. everything else in this channel follows from that

TE

i use bac water even for a single-use vial because it stings less, which is not the reason it exists

thats fine

👍8
EM

the vacuum will pull it in. let it. fighting it is how you get a face full of aerosol

TE

my vial pulled the water in on its own, is that the vacuum or did i do something

📈2🎉6🤝1
TE

swirl it. do not shake it. the foam is denatured protein and you are not getting it back

🤝2😂17
EM

if the maths gives you 13.7 units, change the volume. do not try to hit 13.7

FT

weighing the vial before you reconstitute is the only way to catch an underfill, happy to be corrected