is there any reason not to just always use 2ml
#reconstitution 2025-06-21
Saturday31 messages9 participantstimes are UTC
Highlights from this day
- VialBot — Recon calculator: 20mg in 3ml = 4mg/ml. 16:21
- month_six_me — do i swirl for a specific length of time or just until its clear 18:09
- stack_sceptic — ok so the meniscus thing is real. read it at eye level or you are guessing by a unit either way 18:35
- vat_on_import — benzyl alcohol is what makes it bacteriostatic. it is about 0.9% typically 18:56
- vat_on_import — temperature of the diluent does not matter. how fast you push it in does 19:21
took me a year to stop over-diluting. the measurement error at 4 units is worse than the convenience
weigh it first
Recon calculator: 20mg in 3ml = 4mg/ml.
anyone got a photo of what a properly dissolved vial looks like
lyophilised powder is far more robust than people think. it is the solution that is fragile
how long after reconstituting before its actually dissolved
30mg in 3ml is 10mg/ml, so 5mg is 50 units. half the barrel, feels like a lot but its right
do you let it come to room temp before drawing or draw it cold
give it 8 minutes at room temperature before you decide it has not dissolved
can i add more bac water later to a vial ive already reconstituted
do not add more bac water to an already reconstituted vial, you have lost track of the concentration
pick the volume so your dose is a whole number of units. thats the entire trick
right so you can absolutely just always use 2ml, and honestly most of us should
nobody here should be telling you a dose. we can check your arithmetic, thats a different thing
do i swirl for a specific length of time or just until its clear
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follow up a slightly collapsed cake is cosmetic. a puddle in the bottom is not
ok so the meniscus thing is real. read it at eye level or you are guessing by a unit either way
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bacteriostatic water for anything multi-use. sterile water has no preservative in it at all, happy to be corrected
benzyl alcohol is what makes it bacteriostatic. it is about 0.9% typically
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temperature of the diluent does not matter. how fast you push it in does
10mg vial
+ 1.0ml -> 10.0 mg/ml 0.5mg = 5u 1mg = 10u
+ 2.0ml -> 5.0 mg/ml 0.5mg = 10u 1mg = 20u
+ 2.5ml -> 4.0 mg/ml 0.5mg = 12.5u (dont)
+ 5.0ml -> 2.0 mg/ml 0.5mg = 25u 1mg = 50uswirl it. do not shake it. the foam is denatured protein and you are not getting it back, ill dig out the number
how do you all measure a half unit, or do you just not
too dilute imo
i keep a spare vial of bac water in the fridge purely so i never rush the maths
ok so is it normal for the cake to look slightly collapsed on one side
how much do i lose to dead space with a 31G pin
thats the vacuum
weights-monthly.csv
426 rows · not retained in the public archive
if the powder is on the stopper, tap it down before you add anything. it will still dissolve either way
right so anyone worked out a recon volume that puts 2.4mg on a round unit mark
im getting bubbles in the barrel every single time, what am i doing wrong
update on the earlier thing if i add 2ml to a 10mg vial what concentration am i actually at