pick the volume so your dose is a whole number of units. thats the entire trick
🧪10📉5
#reconstitution 2025-08-12
Tuesday32 messages9 participantstimes are UTC
Highlights from this day
- septum_sal — pick the volume so your dose is a whole number of units. thats the entire trick 22:46
- VialBot — Reminder set. I will post here in 6 days. 22:47
- monoisotopic — the last few units are a real loss. i accept it rather than tilting the vial and drawing air 23:30
- zoya_zeros — does temperature of the bac water matter at all 23:36
Reminder set. I will post here in 6 days.
check your maths
👀7👍10
thats 5mg/ml
not sterile water
sanity check my maths before i pin myself with something stupid
dead space
swirl dont shake
gross powder mass is not net peptide. water and counterion are in that number, ill dig out the number
it should be completely clear with no floaters. cloudy means stop
the jewellery scale is a good habit but calibrate it, they drift badly
write the date on
read it at eye level
i was wrong about this last year: it is fine to draw cold, it just stings more going in
the meniscus thing is real. read it at eye level or you are guessing by a unit either way
i was wrong about this last year: it is fine to draw cold, it just stings more going in
if the maths gives you 13.7 units, change the volume. do not try to hit 13.7
if i add 5ml to a 10mg vial what concentration am i actually at
genuine question aim the diluent at the glass wall, not at the powder. it goes in gentler, thats just me
dead space in a standard insulin syringe hub is small but it is not nothing over a month, ask me again in a month
bac water
thats fine
yep 10 units
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20mg in 2ml is 10mg/ml. 1mg is 10 units, 2mg is 20 units, easy
thats the vacuum
the last few units are a real loss. i accept it rather than tilting the vial and drawing air
10mg vial
+ 1.0ml -> 10.0 mg/ml 0.5mg = 5u 1mg = 10u
+ 2.0ml -> 5.0 mg/ml 0.5mg = 10u 1mg = 20u
+ 2.5ml -> 4.0 mg/ml 0.5mg = 12.5u (dont)
+ 5.0ml -> 2.0 mg/ml 0.5mg = 25u 1mg = 50uthe vacuum will pull it in. let it. fighting it is how you get a face full of aerosol
does temperature of the bac water matter at all
⚠️15✅16👀2
round numbers
nope, redo it
15mg in 3ml is 5mg/ml. same numbers as 10 in 2, just more of it
the jewellery scale is a good habit but calibrate it, they drift badly, we shall see
lyophilised powder is far more robust than people think. it is the solution that is fragile, happy to be corrected
weigh it first