vialroom

#reconstitution 2025-11-14

Friday33 messages7 participantstimes are UTC
Highlights from this day
  • tyne_tracks — sanity check my maths before i pin myself with something stupid pick the volume so your dose is a whole number of units. thats the entire trick 17:29
  • tallinn_taper — the last few units are a real loss. i accept it rather than tilting the vial and drawing air, from memory 18:09
  • vienna_vial — sorry to jump in update from 22 months ago: switched to a single concentration and have not miscalculated since 18:20
  • vienna_vial — is a slightly cloudy solution ever ok or is that always a bin it 18:54
  • VialBot — Assay note: JEEP lot B-0329 reported at 99.2% of label content. 19:22
TT

sanity check my maths before i pin myself with something stupid
pick the volume so your dose is a whole number of units. thats the entire trick

10mg vial
  + 1.0ml  -> 10.0 mg/ml   0.5mg =  5u   1mg = 10u
  + 2.0ml  ->  5.0 mg/ml   0.5mg = 10u   1mg = 20u
  + 2.5ml  ->  4.0 mg/ml   0.5mg = 12.5u (dont)
  + 5.0ml  ->  2.0 mg/ml   0.5mg = 25u   1mg = 50u
TT

if the powder is on the stopper, tap it down before you add anything. it will still dissolve either way

the jewellery scale is a good habit but calibrate it, they drift badly

TT

nobody here should be telling you a dose. we can check your arithmetic, thats a different thing

[edited]
TT

dead space in a standard insulin syringe hub is small but it is not nothing over a month

[edited]

a slightly collapsed cake is cosmetic. a puddle in the bottom is not

10mg in 1ml is 10mg/ml, so 2.5mg is 25 units. thats the one i use

yep 10 units

VV

sorry to jump in update from 22 months ago: switched to a single concentration and have not miscalculated since

TT

a 3ml syringe for the diluent and a u100 for the dose is the setup most people here end up with

update on the earlier thing why does everyone say 2ml specifically and not 1ml

do i swirl for a specific length of time or just until its clear

gross powder mass is not net peptide. water and counterion are in that number

PM

10mg in 2ml is 5mg/ml. so 0.5mg is 0.1ml which is 10 units on a u100

is a slightly cloudy solution ever ok or is that always a bin it

📉6💀10
VB

Assay note: JEEP lot B-0329 reported at 99.2% of label content.

VV

coming back to this benzyl alcohol is what makes it bacteriostatic. it is about 0.9% typically

PM

is the meniscus reading actually a real source of error or am i overthinking

PM

whats the smallest volume you would trust yourself to measure on a u100

TT

pick the volume so your dose is a whole number of units. thats the entire trick

TT

give it 7 minutes at room temperature before you decide it has not dissolved, ill dig out the number

i was wrong about this last year: it is fine to draw cold, it just stings more going in