is the meniscus reading actually a real source of error or am i overthinking
#reconstitution 2025-12-19
Friday16 messages5 participantstimes are UTC
Highlights from this day
- taste_changed — swirl it. do not shake it. the foam is denatured protein and you are not getting it back 15:59
- taste_changed — one unit is 0.01ml. 100 units is 1ml. everything else in this channel follows from that 16:43
- swirl_not_shake — gross powder mass is not net peptide. water and counterion are in that number, from memory 16:44
- taste_changed — the meniscus thing is real. read it at eye level or you are guessing by a unit either way, i have it written down somewhere 19:21
is it normal for the cake to look slightly collapsed on one side
i write the mg/ml on the vial with a sharpie the moment i reconstitute it, we shall see
read it at eye level
not sterile water
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yep 10 units
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sanity check my maths before i pin myself with something stupid
swirl it. do not shake it. the foam is denatured protein and you are not getting it back
one unit is 0.01ml. 100 units is 1ml. everything else in this channel follows from that
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gross powder mass is not net peptide. water and counterion are in that number, from memory
20mg in 2ml is 10mg/ml. 1mg is 10 units, 2mg is 20 units, easy
thats fine
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give it 8 minutes at room temperature before you decide it has not dissolved
is a slightly cloudy solution ever ok or is that always a bin it
whats the smallest volume you would trust yourself to measure on a u100
the meniscus thing is real. read it at eye level or you are guessing by a unit either way, i have it written down somewhere