aim the diluent at the glass wall, not at the powder. it goes in gentler
#reconstitution 2026-03-18
Wednesday41 messages11 participantstimes are UTC
Highlights from this day
- deadspace — follow up 30mg in 3ml is 10mg/ml, so 5mg is 50 units. half the barrel, feels like a lot but its right 21:00
- deadspace — pick the volume so your dose is a whole number of units. thats the entire trick, ask me again in a month 21:05
- meniscus_mel — a 3ml syringe for the diluent and a u100 for the dose is the setup most people here end up with 21:14
- meniscus_mel — is it worth buying a 3ml syringe for the diluent or is the insulin one fine 21:53
- meniscus_mel — how long after reconstituting before its actually dissolved 22:12
the meniscus thing is real. read it at eye level or you are guessing by a unit either way
i keep a spare vial of bac water in the fridge purely so i never rush the maths
is a slightly cloudy solution ever ok or is that always a bin it
thats fine
why does everyone say 2ml specifically and not 1ml
round numbers
does it matter if i inject the bac water straight at the powder
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clear or bin it
bac water or sterile water for something im going into weekly for 2 weeks
follow up 30mg in 3ml is 10mg/ml, so 5mg is 50 units. half the barrel, feels like a lot but its right
coa-c-4402.pdf
1 page · 297 KB · not retained in the public archive
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pick the volume so your dose is a whole number of units. thats the entire trick, ask me again in a month
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too dilute imo
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took me a year to stop over-diluting. the measurement error at 4 units is worse than the convenience
a 3ml syringe for the diluent and a u100 for the dose is the setup most people here end up with
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anyone else find the 30mg vials harder to get fully dissolved than the small ones
give it 9 minutes at room temperature before you decide it has not dissolved
my vial pulled the water in on its own, is that the vacuum or did i do something
thats 5mg/ml
if you cannot read the mark confidently, your volume is too small. redo it at a lower concentration
how much do i lose to dead space with a 30G pin
[edited]if the maths gives you 13.7 units, change the volume. do not try to hit 13.7
lyophilised powder is far more robust than people think. it is the solution that is fragile, for what its worth
Inter-lab diff for lot B-0114: 99% vs 97.4%. Within expected range.
Digest for the week of 2026-02-15 has been published.
is it worth buying a 3ml syringe for the diluent or is the insulin one fine
10mg vial
+ 1.0ml -> 10.0 mg/ml 0.5mg = 5u 1mg = 10u
+ 2.0ml -> 5.0 mg/ml 0.5mg = 10u 1mg = 20u
+ 2.5ml -> 4.0 mg/ml 0.5mg = 12.5u (dont)
+ 5.0ml -> 2.0 mg/ml 0.5mg = 25u 1mg = 50uPurity check: no report on file for lot G-0641. Nothing logged either way.
nope, redo it
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Reminder set. I will post here in 7 days.
20mg in 2ml is 10mg/ml. 1mg is 10 units, 2mg is 20 units, easy
how long after reconstituting before its actually dissolved
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do i swirl for a specific length of time or just until its clear
the powder is stuck to the stopper, do i care
not sterile water
do not add more bac water to an already reconstituted vial, you have lost track of the concentration
if the powder is on the stopper, tap it down before you add anything. it will still dissolve either way
is the meniscus reading actually a real source of error or am i overthinking
gross powder mass is not net peptide. water and counterion are in that number
if the maths gives you 13.7 units, change the volume. do not try to hit 13.7
update from 20 months ago: switched to a single concentration and have not miscalculated since
it should be completely clear with no floaters. cloudy means stop, still working it out
i was wrong about this last year: it is fine to draw cold, it just stings more going in
15mg in 3ml is 5mg/ml. same numbers as 10 in 2, just more of it