anyone worked out a recon volume that puts 7.5mg on a round unit mark
#reconstitution 2026-04-15
Wednesday48 messages11 participantstimes are UTC
Highlights from this day
- aliquot_ada — lyophilised powder is far more robust than people think. it is the solution that is fragile 17:40
- bea_reconstitutes — quick one swirl it. do not shake it. the foam is denatured protein and you are not getting it back 18:40
- gorse_gains — i was wrong about this last year: it is fine to draw cold, it just stings more going in, ill dig out the number 19:17
- bea_reconstitutes — quick one my 10mg vial weighed 11.4g on the jewellery scale, is that wrong 20:07
- jute_journals — dead space in a standard insulin syringe hub is small but it is not nothing over a month lyophilised powder is far more robust than people think. it is the solution… 20:32
i use bac water even for a single-use vial because it stings less, which is not the reason it exists
fridge-thermometer.png
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one unit is 0.01ml
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im getting bubbles in the barrel every single time, what am i doing wrong
if you draw up with a bigger pin and inject with a fresh one you lose a bit more to dead space
the powder is stuck to the stopper, do i care
i swirl for about thirty seconds and then leave it. never had one fail to dissolve
do i swirl for a specific length of time or just until its clear
genuine question benzyl alcohol is what makes it bacteriostatic. it is about 0.9% typically
10mg in 1ml is 10mg/ml, so 2.5mg is 25 units. thats the one i use
why does everyone say 2ml specifically and not 1ml
[edited]lot-log.csv
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nobody here should be telling you a dose. we can check your arithmetic, thats a different thing
if you cannot read the mark confidently, your volume is too small. redo it at a lower concentration
a slightly collapsed cake is cosmetic. a puddle in the bottom is not
i keep a spare vial of bac water in the fridge purely so i never rush the maths
is it normal for the cake to look slightly collapsed on one side
thats 5mg/ml
weigh it first
read it at eye level
update on the earlier thing the meniscus thing is real. read it at eye level or you are guessing by a unit either way, from memory
my rule is one concentration for everything so i never have to think about it at 7am
nope, redo it
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lyophilised powder is far more robust than people think. it is the solution that is fragile
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weighing the vial before you reconstitute is the only way to catch an underfill, i think
aim the diluent at the glass wall, not at the powder. it goes in gentler
gross powder mass is not net peptide. water and counterion are in that number
[edited]5mg in 2ml gives 2.5mg/ml, 0.25mg is 10 units. good for a starting dose
i have never once regretted using a rounder number and a slightly bigger volume
10mg vial
+ 1.0ml -> 10.0 mg/ml 0.5mg = 5u 1mg = 10u
+ 2.0ml -> 5.0 mg/ml 0.5mg = 10u 1mg = 20u
+ 2.5ml -> 4.0 mg/ml 0.5mg = 12.5u (dont)
+ 5.0ml -> 2.0 mg/ml 0.5mg = 25u 1mg = 50uhow much do i lose to dead space with a 29G pin
Inter-lab diff for lot B-0329: 97.4% vs 98.6%. Within expected range.
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anyone got a photo of what a properly dissolved vial looks like
you can absolutely just always use 2ml, and honestly most of us should, someone check my working
bacteriostatic water for anything multi-use. sterile water has no preservative in it at all
it foamed. is it ruined
how do you all measure a half unit, or do you just not
quick one swirl it. do not shake it. the foam is denatured protein and you are not getting it back
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10mg in 1ml is 10mg/ml, so 2.5mg is 25 units. thats the one i use
yep 10 units
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write the date on
15mg in 3ml is 5mg/ml. same numbers as 10 in 2, just more of it, your mileage will differ
i was wrong about this last year: it is fine to draw cold, it just stings more going in, ill dig out the number
U-100 syringe: 1 unit = 0.01 ml
10u = 0.10 ml
25u = 0.25 ml
50u = 0.50 ml
100u = 1.00 ml
mg drawn = ml drawn x mg/mlif the maths gives you 13.7 units, change the volume. do not try to hit 13.7
update from 8 months ago: switched to a single concentration and have not miscalculated since
i write the mg/ml on the vial with a sharpie the moment i reconstitute it
bac water
quick one my 10mg vial weighed 11.4g on the jewellery scale, is that wrong
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bac water or sterile water for something im going into weekly for 3 weeks
if i add 2.5ml to a 20mg vial what concentration am i actually at
*2ml not 2.5
dead space in a standard insulin syringe hub is small but it is not nothing over a month
lyophilised powder is far more robust than people think. it is the solution that is fragile
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