vialroom

#reconstitution 2026-06-16

Tuesday46 messages7 participantstimes are UTC
Highlights from this day
  • ari_arrives — if i add 0.5ml to a 15mg vial what concentration am i actually at 17:27
  • cold_chain_cmdr — for the archive my rule is one concentration for everything so i never have to think about it at 7am, for what its worth 18:53
  • vat_on_import — bacteriostatic water for anything multi-use. sterile water has no preservative in it at all, ymmv 19:23
  • ivy_injects — slightly off topic but the powder is stuck to the stopper, do i care 21:06
  • clothes_size_cass — what do you do with the last 25 units when you cant draw them cleanly 21:28
AA

nope, redo it

10mg vial
  + 1.0ml  -> 10.0 mg/ml   0.5mg =  5u   1mg = 10u
  + 2.0ml  ->  5.0 mg/ml   0.5mg = 10u   1mg = 20u
  + 2.5ml  ->  4.0 mg/ml   0.5mg = 12.5u (dont)
  + 5.0ml  ->  2.0 mg/ml   0.5mg = 25u   1mg = 50u

took me a year to stop over-diluting. the measurement error at 4 units is worse than the convenience

if i add 0.5ml to a 15mg vial what concentration am i actually at

⚠️1815

check your maths

[edited]
VO

slightly off topic but benzyl alcohol is what makes it bacteriostatic. it is about 0.9% typically

VO

temperature of the diluent does not matter. how fast you push it in does

AA

if you draw up with a bigger pin and inject with a fresh one you lose a bit more to dead space

CC

if the powder is on the stopper, tap it down before you add anything. it will still dissolve either way

VO

update from 7 months ago: switched to a single concentration and have not miscalculated since, not advice obviously

AA

5mg in 2ml gives 2.5mg/ml, 0.25mg is 10 units. good for a starting dose, still working it out

CC

for the archive my rule is one concentration for everything so i never have to think about it at 7am, for what its worth

CC

do not add more bac water to an already reconstituted vial, you have lost track of the concentration

CC

i use bac water even for a single-use vial because it stings less, which is not the reason it exists

if you cannot read the mark confidently, your volume is too small. redo it at a lower concentration

nobody here should be telling you a dose. we can check your arithmetic, thats a different thing

i write the mg/ml on the vial with a sharpie the moment i reconstitute it

follow up i have never once regretted using a rounder number and a slightly bigger volume, ymmv

VO

bacteriostatic water for anything multi-use. sterile water has no preservative in it at all, ymmv

🎉13👍9

a 3ml syringe for the diluent and a u100 for the dose is the setup most people here end up with

VO

follow up gross powder mass is not net peptide. water and counterion are in that number

the meniscus thing is real. read it at eye level or you are guessing by a unit either way

dead space in a standard insulin syringe hub is small but it is not nothing over a month

[edited]
SH

for the archive 30mg in 3ml is 10mg/ml, so 5mg is 50 units. half the barrel, feels like a lot but its right

if the maths gives you 13.7 units, change the volume. do not try to hit 13.7

anyone got a photo of what a properly dissolved vial looks like

AA

lyophilised powder is far more robust than people think. it is the solution that is fragile

i swirl for about thirty seconds and then leave it. never had one fail to dissolve

II

im getting bubbles in the barrel every single time, what am i doing wrong

give it 9 minutes at room temperature before you decide it has not dissolved

i was wrong about this last year: it is fine to draw cold, it just stings more going in, thats one data point

too dilute imo

🧪3⚠️2🙏4

one unit is 0.01ml. 100 units is 1ml. everything else in this channel follows from that

II

my vial pulled the water in on its own, is that the vacuum or did i do something

II

weighing the vial before you reconstitute is the only way to catch an underfill

II

you can absolutely just always use 2ml, and honestly most of us should