i was wrong about this last year: it is fine to draw cold, it just stings more going in, thats one data point
[edited]#reconstitution 2026-06-29
Monday65 messages14 participantstimes are UTC
Highlights from this day
- hydrate_hana — the meniscus thing is real. read it at eye level or you are guessing by a unit either way 13:24
- non_scale_win — is it worth buying a 3ml syringe for the diluent or is the insulin one fine 16:34
- mg_per_ml — i swirl for about thirty seconds and then leave it. never had one fail to dissolve 18:25
- mg_per_ml — 20mg in 2ml is 10mg/ml. 1mg is 10 units, 2mg is 20 units, easy 18:41
round numbers
does benzyl alcohol matter if im finishing the vial in one go
thats fine
if the maths gives you 13.7 units, change the volume. do not try to hit 13.7
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i keep a spare vial of bac water in the fridge purely so i never rush the maths
if you cannot read the mark confidently, your volume is too small. redo it at a lower concentration
lyophilised powder is far more robust than people think. it is the solution that is fragile
benzyl alcohol is what makes it bacteriostatic. it is about 0.9% typically
thats 5mg/ml
check your maths
you can absolutely just always use 2ml, and honestly most of us should
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coming back after 17 months, has the pinned recon volume advice changed
if the powder is on the stopper, tap it down before you add anything. it will still dissolve either way
weighing the vial before you reconstitute is the only way to catch an underfill
10mg in 1ml is 10mg/ml, so 2.5mg is 25 units. thats the one i use
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my rule is one concentration for everything so i never have to think about it at 7am
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can i add more bac water later to a vial ive already reconstituted
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whats the smallest volume you would trust yourself to measure on a u100
why does everyone say 2ml specifically and not 1ml
gross powder mass is not net peptide. water and counterion are in that number
the meniscus thing is real. read it at eye level or you are guessing by a unit either way
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thats the vacuum
sanity check my maths before i pin myself with something stupid
[edited]fridge-thermometer.png
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the last few units are a real loss. i accept it rather than tilting the vial and drawing air
weights-monthly.csv
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aim the diluent at the glass wall, not at the powder. it goes in gentler
im getting bubbles in the barrel every single time, what am i doing wrong
the jewellery scale is a good habit but calibrate it, they drift badly
sorry to jump in 10mg in 1ml is 10mg/ml, so 2.5mg is 25 units. thats the one i use
swirl it. do not shake it. the foam is denatured protein and you are not getting it back
how long after reconstituting before its actually dissolved
not sterile water
do you draw up with the same pin you inject with
do i swirl for a specific length of time or just until its clear
5mg in 2ml gives 2.5mg/ml, 0.25mg is 10 units. good for a starting dose
write the date on
clear or bin it
New independent result logged — GL Biochem, lot F-1330, purity 99% (VendorInvestigate).
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if you draw up with a bigger pin and inject with a fresh one you lose a bit more to dead space
my vial pulled the water in on its own, is that the vacuum or did i do something
10mg in 2ml is 5mg/ml. so 0.5mg is 0.1ml which is 10 units on a u100
gross powder mass is not net peptide. water and counterion are in that number
weigh it first
took me a year to stop over-diluting. the measurement error at 4 units is worse than the convenience
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right so temperature of the diluent does not matter. how fast you push it in does
the vacuum will pull it in. let it. fighting it is how you get a face full of aerosol, ask me again in a month
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update from 21 months ago: switched to a single concentration and have not miscalculated since, happy to be corrected
swirl dont shake
it should be completely clear with no floaters. cloudy means stop
is it worth buying a 3ml syringe for the diluent or is the insulin one fine
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i have never once regretted using a rounder number and a slightly bigger volume, from memory
is there any reason not to just always use 2ml
label the date too. you will not remember which vial is which by week 28
dead space
pick the volume so your dose is a whole number of units. thats the entire trick, i think
a 3ml syringe for the diluent and a u100 for the dose is the setup most people here end up with
nope, redo it
U-100 syringe: 1 unit = 0.01 ml
10u = 0.10 ml
25u = 0.25 ml
50u = 0.50 ml
100u = 1.00 ml
mg drawn = ml drawn x mg/mlyep 10 units
i swirl for about thirty seconds and then leave it. never had one fail to dissolve
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20mg in 2ml is 10mg/ml. 1mg is 10 units, 2mg is 20 units, easy
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anyone else find the 20mg vials harder to get fully dissolved than the small ones
the powder is stuck to the stopper, do i care
Reminder for vienna_vial: dose day is today. Set 5 days ago.
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nobody here should be telling you a dose. we can check your arithmetic, thats a different thing
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one unit is 0.01ml
give it 8 minutes at room temperature before you decide it has not dissolved
is it normal for the cake to look slightly collapsed on one side