Topic
Degradants
Page 12 of 13 — Deamidation at plus one dalton, oxidation at plus sixteen, truncation sequences, and what a warm transit actually does.
Messages — page 12 of 13
LC_MS_Lenaplus 16 is oxidation#hplc-massspec · 2026-05-19LC_MS_Lenahow would a truncation sequence show up#hplc-massspec · 2026-05-19dill_dosesupdate on the earlier thing how would a truncation sequence show up#hplc-massspec · 2026-05-20drawup_deea warm transit can put a visible degradant peak on a chromatogram. that is what 12 days at ambient does#hplc-massspec · 2026-05-23monoisotopicoxidation is about plus 15.99 Da and is usually methionine or tryptophan 214nm sees the amide backbone so it sees everything.…#hplc-massspec · 2026-05-23thirty_min_waithow would a truncation sequence show up#hplc-massspec · 2026-05-23void_volumeplus 0.98 is deamidation#hplc-massspec · 2026-05-24yara_yieldsdeamidation is about plus 0.98 Da. it is not a rounding error, it is a degradant#hplc-massspec · 2026-05-24void_volumeoxidation is about plus 15.99 Da and is usually methionine or tryptophan, i think#hplc-massspec · 2026-05-24void_volumeplus sixteen, is that always oxidation#hplc-massspec · 2026-05-24sgp_subqplus 0.98 is deamidation#hplc-massspec · 2026-05-25apob_over_ldloxidation is about plus 15.99 Da and is usually methionine or tryptophan#hplc-massspec · 2026-05-29apob_over_ldla warm transit can put a visible degradant peak on a chromatogram. that is what 17 days at ambient does, ymmv#hplc-massspec · 2026-05-29tarpit_tamdeamidation is about plus 0.98 Da. it is not a rounding error, it is a degradant#hplc-massspec · 2026-05-29tarpit_tamplus 0.98 is deamidation#hplc-massspec · 2026-05-29identity_shiftplus 16 is oxidation#hplc-massspec · 2026-05-29identity_shiftoxidation is about plus 15.99 Da and is usually methionine or tryptophan#hplc-massspec · 2026-05-30ivy_injectsa warm transit can put a visible degradant peak on a chromatogram. that is what 26 days at ambient does#hplc-massspec · 2026-05-30lead_time_larsslightly off topic but truncations show as a mass deficit matching one or more residues. you need the sequence to say which#hplc-massspec · 2026-05-30lead_time_larsplus 16 is oxidation#hplc-massspec · 2026-05-30fridge_thermotruncations show as a mass deficit matching one or more residues. you need the sequence to say which#hplc-massspec · 2026-05-31tail_factoroxidation is about plus 15.99 Da and is usually methionine or tryptophan#hplc-massspec · 2026-05-31chlorhexplus sixteen, is that always oxidation#hplc-massspec · 2026-05-31back_from_awaydeamidation is about plus 0.98 Da. it is not a rounding error, it is a degradant, ill dig out the number truncations show as a…#hplc-massspec · 2026-05-31void_volumea warm transit can put a visible degradant peak on a chromatogram. that is what 26 days at ambient does#hplc-massspec · 2026-05-31back_from_awaycoming back to this how would a truncation sequence show up#hplc-massspec · 2026-05-31back_from_awayplus 16 is oxidation#hplc-massspec · 2026-05-31monoisotopicdeamidation is about plus 0.98 Da. it is not a rounding error, it is a degradant#hplc-massspec · 2026-06-03void_volumeplus sixteen, is that always oxidation#hplc-massspec · 2026-06-03deamidationshipping can degrade a peptide. shipping does not remove 19% of the mass from a sealed vial#test-results · 2026-06-03sedge_sourcesa warm transit can put a visible degradant peak on a chromatogram. that is what 7 days at ambient does#hplc-massspec · 2026-06-04sedge_sourcesoxidation is about plus 15.99 Da and is usually methionine or tryptophan, ill dig out the number#hplc-massspec · 2026-06-04sedge_sourcesdeamidation is about plus 0.98 Da. it is not a rounding error, it is a degradant#hplc-massspec · 2026-06-04salt_bridgeplus sixteen, is that always oxidation#hplc-massspec · 2026-06-04reship_ritaplus 16 is oxidation#hplc-massspec · 2026-06-06pia_plateaustruncations show as a mass deficit matching one or more residues. you need the sequence to say which, your mileage will differ#hplc-massspec · 2026-06-06vienna_vialhow would a truncation sequence show up#hplc-massspec · 2026-06-08vienna_vialplus sixteen, is that always oxidation#hplc-massspec · 2026-06-08baseline_driftplus 16 is oxidation#hplc-massspec · 2026-06-08HPLC_Hanksorry to jump in a warm transit can put a visible degradant peak on a chromatogram. that is what 8 days at ambient does#hplc-massspec · 2026-06-08early_satietydeamidation is about plus 0.98 Da. it is not a rounding error, it is a degradant#hplc-massspec · 2026-06-08per_mg_peteoxidation is about plus 15.99 Da and is usually methionine or tryptophan#hplc-massspec · 2026-06-12void_volumeplus 0.98 is deamidation#hplc-massspec · 2026-06-12void_volumegenuine question a warm transit can put a visible degradant peak on a chromatogram. that is what 5 days at ambient does#hplc-massspec · 2026-06-12site_itchplus 0.98 is deamidation#hplc-massspec · 2026-06-13site_itchoxidation is about plus 15.99 Da and is usually methionine or tryptophan, ill dig out the number#hplc-massspec · 2026-06-13retention_timeplus 0.98 is deamidation#hplc-massspec · 2026-06-14peak_splitplus sixteen, is that always oxidation#hplc-massspec · 2026-06-14peak_splitplus 16 is oxidation#hplc-massspec · 2026-06-14bp_down_bexoxidation is about plus 15.99 Da and is usually methionine or tryptophan#hplc-massspec · 2026-06-14split_shipmentcoming back to this truncations show as a mass deficit matching one or more residues. you need the sequence to say which#hplc-massspec · 2026-06-14gorse_gainshow would a truncation sequence show up#hplc-massspec · 2026-06-14plum_plotshow would a truncation sequence show up#hplc-massspec · 2026-06-16early_satietyplus 16 is oxidation#hplc-massspec · 2026-06-16mira_pinsoxidation is about plus 15.99 Da and is usually methionine or tryptophan you need more sample for identity than for purity.…#hplc-massspec · 2026-06-16mira_pinsa warm transit can put a visible degradant peak on a chromatogram. that is what 24 days at ambient does#hplc-massspec · 2026-06-16swirl_not_shakeplus 16 is oxidation#hplc-massspec · 2026-06-17swirl_not_shaketruncations show as a mass deficit matching one or more residues. you need the sequence to say which, i have it written down…#hplc-massspec · 2026-06-17orfor_watchdeamidation is about plus 0.98 Da. it is not a rounding error, it is a degradant#hplc-massspec · 2026-06-17orfor_watchplus 0.98 is deamidation#hplc-massspec · 2026-06-17