Topic
Degradants
Page 2 of 13 — Deamidation at plus one dalton, oxidation at plus sixteen, truncation sequences, and what a warm transit actually does.
Messages — page 2 of 13
blank_rundeamidation is about plus 0.98 Da. it is not a rounding error, it is a degradant#hplc-massspec · 2025-03-30blank_runa warm transit can put a visible degradant peak on a chromatogram. that is what 19 days at ambient does#hplc-massspec · 2025-03-30blank_runplus 0.98 is deamidation#hplc-massspec · 2025-03-30void_volumeplus 0.98 is deamidation#hplc-massspec · 2025-04-01madrid_mgdeamidation is about plus 0.98 Da. it is not a rounding error, it is a degradant#hplc-massspec · 2025-04-01ruo_onlyplus sixteen, is that always oxidation#hplc-massspec · 2025-04-08madrid_mgplus 0.98 is deamidation#hplc-massspec · 2025-04-08gall_bladder_gava warm transit can put a visible degradant peak on a chromatogram. that is what 20 days at ambient does, i think#hplc-massspec · 2025-04-08madrid_mgplus 16 is oxidation#hplc-massspec · 2025-04-08cat_on_the_trayoxidation is about plus 15.99 Da and is usually methionine or tryptophan#hplc-massspec · 2025-04-08vialkeepertruncations show as a mass deficit matching one or more residues. you need the sequence to say which#hplc-massspec · 2025-04-12typosquat_tayoxidation is about plus 15.99 Da and is usually methionine or tryptophan#hplc-massspec · 2025-04-12vialkeeperhow would a truncation sequence show up#hplc-massspec · 2025-04-12cold_chain_cmdrdeamidation is about plus 0.98 Da. it is not a rounding error, it is a degradant#hplc-massspec · 2025-04-14vial_ledgerplus 0.98 is deamidation#hplc-massspec · 2025-04-14alt_ast_aliplus 0.98 is deamidation#hplc-massspec · 2025-04-15alt_ast_aliplus 16 is oxidation#hplc-massspec · 2025-04-15freeze_thawa warm transit can put a visible degradant peak on a chromatogram. that is what 13 days at ambient does#hplc-massspec · 2025-04-15amylin_amyplus 0.98 is deamidation#hplc-massspec · 2025-04-20salt_bridgethat pattern is very familiar. the earlier one is usually deamidation, the later one is often oxidation#hplc-massspec · 2025-04-20salt_bridgedeamidation converts an asparagine to an aspartate and adds about 1 Da. one dalton on a 4000 Da peptide is invisible on a…#hplc-massspec · 2025-04-20vial_ledgerwhich is why the deamidated peak usually sits at RRT just under 1.00 and people misread it as an unreacted intermediate#hplc-massspec · 2025-04-20week_zerowhat about the truncation thing, whats a deletion sequence#hplc-massspec · 2025-04-20week_zeroone thing i still do not get. if my vial degraded, is it less potent or differently potent#hplc-massspec · 2025-04-20salt_bridgefor a deamidated or oxidised fraction the honest answer is we do not know the activity of that fraction from a chromatogram.…#hplc-massspec · 2025-04-20deamidationupdate on the earlier thing a warm transit can put a visible degradant peak on a chromatogram. that is what 18 days at ambient…#hplc-massspec · 2025-04-26truncation_trudeamidation is about plus 0.98 Da. it is not a rounding error, it is a degradant#hplc-massspec · 2025-04-26alcohol_hitsplus 0.98 is deamidation#hplc-massspec · 2025-04-26LC_MS_Lenaunrelated but oxidation is about plus 15.99 Da and is usually methionine or tryptophan#hplc-massspec · 2025-04-26HPLC_Hankplus 0.98 is deamidation#hplc-massspec · 2025-04-28LC_MS_Lenadeamidation is about plus 0.98 Da. it is not a rounding error, it is a degradant#hplc-massspec · 2025-05-01LC_MS_Lenahow would a truncation sequence show up#hplc-massspec · 2025-05-01yyz_viala warm transit can put a visible degradant peak on a chromatogram. that is what 21 days at ambient does#hplc-massspec · 2025-05-01provincial_pattruncations show as a mass deficit matching one or more residues. you need the sequence to say which#hplc-massspec · 2025-05-03area_percentplus 16 is oxidation#hplc-massspec · 2025-05-03area_percentoxidation is about plus 15.99 Da and is usually methionine or tryptophan#hplc-massspec · 2025-05-03week_three_walla warm transit can put a visible degradant peak on a chromatogram. that is what 13 days at ambient does#hplc-massspec · 2025-05-04week_three_wallplus sixteen, is that always oxidation#hplc-massspec · 2025-05-04HPLC_Hankdeamidation is about plus 0.98 Da. it is not a rounding error, it is a degradant#hplc-massspec · 2025-05-04combo_caiplus sixteen, is that always oxidation#hplc-massspec · 2025-05-07combo_caioxidation is about plus 15.99 Da and is usually methionine or tryptophan#hplc-massspec · 2025-05-07sulphur_burphow would a truncation sequence show up#hplc-massspec · 2025-05-07half_life_hala warm transit can put a visible degradant peak on a chromatogram. that is what 11 days at ambient does#hplc-massspec · 2025-05-10deamidationplus 16 is oxidation#hplc-massspec · 2025-05-10hiccup_hollisoxidation is about plus 15.99 Da and is usually methionine or tryptophan#hplc-massspec · 2025-05-10manila_meniscustruncations show as a mass deficit matching one or more residues. you need the sequence to say which semaglutide is about…#hplc-massspec · 2025-05-10half_life_haldeamidation is about plus 0.98 Da. it is not a rounding error, it is a degradant#hplc-massspec · 2025-05-10seven_five_sweeta warm transit can put a visible degradant peak on a chromatogram. that is what 24 days at ambient does#hplc-massspec · 2025-05-13coa_or_copeplus 0.98 is deamidation#hplc-massspec · 2025-05-13mcg_not_mgdeamidation is about plus 0.98 Da. it is not a rounding error, it is a degradant#hplc-massspec · 2025-05-13cold_start_cysorry to jump in plus sixteen, is that always oxidation#hplc-massspec · 2025-05-13oasis_olahow would a truncation sequence show up#hplc-massspec · 2025-05-13oasis_olaplus 16 is oxidation#hplc-massspec · 2025-05-13karl_fischerplus 16 is oxidation#hplc-massspec · 2025-05-16acetate_ashplus 0.98 is deamidation#hplc-massspec · 2025-05-16area_percentdeamidation is about plus 0.98 Da. it is not a rounding error, it is a degradant#hplc-massspec · 2025-05-16freeze_thawright so oxidation is about plus 15.99 Da and is usually methionine or tryptophan#hplc-massspec · 2025-05-16freeze_thawa warm transit can put a visible degradant peak on a chromatogram. that is what 24 days at ambient does#hplc-massspec · 2025-05-16freeze_thawcoming back to this how would a truncation sequence show up#hplc-massspec · 2025-05-16void_volumeupdate on the earlier thing plus sixteen, is that always oxidation#hplc-massspec · 2025-05-25